神戸大学附属図書館デジタルアーカイブ
https://doi.org/10.24546/00522838

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メタデータID
00522838
アクセス権
open access
出版タイプ
Version of Record
タイトル
Analysis of phosphatidylcholine oxidation products in human plasma using quadrupole time-of flight mass spectrometry
著者
Adachi, Junko ; Asano, Migiwa ; Yoshioka, Naoki ; Nushida, Hideyuki ; Ueno, Yasuhiro
著者名
Adachi, Junko
著者名
Asano, Migiwa
著者名
Yoshioka, Naoki
著者名
Nushida, Hideyuki
著者名
Ueno, Yasuhiro
収録物名
The Kobe journal of the medical sciences
巻(号)
52(5)
ページ
127-140
出版者
神戸大学医学部
Kobe University School of Medicine
刊行日
2006-03
公開日
2007-07-20
抄録
We report here an application of the previous method for the analysis ofphosphatidylcholine (PC) and lysophosphatidylcholine (lysoPC) oxidation products inhuman plasma using quadrupole time of flight (Q-TOF) mass spectrometry withelectrospray ionization. We separated these products using an HPLC C8 column witha gradient of methanol and 10 mM aqueous ammonium acetate. Monohydroperoxides,epoxyhydroxy derivatives, oxo derivatives, and trihydroxides of palmitoyl-linoleoyl(C16:0/C18:2) PC and stearoyl-linoleoyl (C18:0/C18:2) PC were detected mainly asMH+ and [M+Na]+ ions in the plasma of alcoholic patients. Using standard syntheticPC-OH (C16:0/C18:2-OH), the lipid extract component was identified as(C16:0/C18:2-OH) PC based on the product ions of ESI-MS-MS. Using standardsynthetic PCOOH (C16:0/C18:2-OOH) as a reference, the PCOOH concentration inplasma was quantified. Two oxidatively modified lysoPCs were also detected. This isthe first report showing the presence of epoxyhydroxy derivatives,monohydroperoxides, oxo derivatives, and trihydroxides of (C16:0/C18:2) PC and(C18:0/C18:2) PC, and PC-OH (C16:0/C18:0-OH) in human plasma.
キーワード
plasma
LC-MS
phosphatidylcholine
oxidation products
カテゴリ
The Kobe journal of the medical sciences ＞ 52巻 ＞ 52巻5号（2006）
紀要論文