神戸大学附属図書館デジタルアーカイブ
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学内刊行物
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https://doi.org/10.24546/81000096
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2024-03-28
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81000096 (fulltext)
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メタデータID
81000096
アクセス権
open access
出版タイプ
Version of Record
タイトル
Clinical and Histopathological Analysis of Healing Process of Intraoral Reconstruction with ex vivo Produced Oral Mucosa Equivalent
著者
Hotta, Takeshi ; Yokoo, Satoshi ; Terashi, Hiroto ; Komori, Takahide
著者名
Hotta, Takeshi
著者名
Yokoo, Satoshi
著者ID
A0056
研究者ID
1000080217421
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/profile/ja.1431d96c128be803520e17560c007669.html
著者名
Terashi, Hiroto
寺師, 浩人
テラシ, ヒロト
所属機関名
医学部附属病院
著者ID
A0121
研究者ID
1000050251294
著者名
Komori, Takahide
古森, 孝英
コモリ, タカヒデ
所属機関名
医学研究科
収録物名
The Kobe journal of the medical sciences
巻(号)
53(1)
ページ
1-14
出版者
神戸大学医学部
Kobe University School of Medicine
刊行日
2007-02
公開日
2008-02-20
抄録
We fabricated ex vivo produced oral mucosa equivalent (EVPOME) from patients'oral mucosal keratinocytes without using animal-derived serum or feeder layer cells.To confirm the clinical benefits of 1) early initiation of epithelialization, 2) a shortperiod until complete healing and 3) negligible scar contracture, the mechanism ofwound healing after EVPOME transplantation for oral mucosal defects was analyzedhistopathologically. Transplantation was performed on 15 patients (eight men andseven women; aged between 51 and 76 years, mean, 66.6 years). Two patients hadsquamous cell carcinoma of the tongue, nine had leukoplakia (four in the tongue only,two in the gingiva only, one in the buccal mucosa, and two in two or more areas), andfour had hypoplasia in the alveolar ridge. The mean interval between punch-biopsy forthe fabrication of EVPOME and its transplantation for the reconstruction of oralmucosal defects was 28.5 days, by which time EVPOME with a mean size of 6.5 cm^2and a cell count of 8.6 × 10^5 could be obtained. The underlying disease, past history,and smoking history of the patients did not constitute negative factors for EVPOMEfabrication. About 10 days after transplantation, EVPOME began uniting with thesurrounding epithelium. The mean duration required for the wound to be completelycovered (28.2 days) was much shorter than after transplantation of only an acellularallogenic dermal matrix (AlloDerm○!R), and showed only slight scar formation, similarto that observed after artificial dermis (Terdermis○!R) transplantation. Presence oflaminin-1, 5 and type IV collagen in the basement membrane of EVPOME wasconfirmed, and the arrangement and positioning of keratinocytes were preservedduring the degradation of perlecan and anchoring fibrils (type VII collagen) forremodeling, i.e., the period of the most active remodeling of EVPOME transplantation.Only a few fibroblasts were observed in the lamina propria during this period,suggesting that keratinocyte-derived cytokines, rather than fibroblast-derivedcytokines, play an important role in the early stages of mucosal wound healing afterEVPOME transplantation. The efficacy of EVPOME is associated with closely relatedto the presence of the keratinocyte-derived system and the usefulness of AlloDerm○!R thatsustains keratinocytes.
キーワード
Oral mucosa equivalent
oral keratinocyte
acellular allogeneic dermal matrix wound healing
カテゴリ
医学研究科
医学部附属病院
The Kobe journal of the medical sciences
>
53巻
>
53巻1号(2007-02)
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資源タイプ
departmental bulletin paper
言語
English (英語)
ISSN
0023-2513
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NCID
AA00711740
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関連情報
NAID
110006630179
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URI
http://www.med.kobe-u.ac.jp/journal/contents.html
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