神戸大学附属図書館デジタルアーカイブ
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https://doi.org/10.24546/81003264
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2024-04-20
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81003264 (fulltext)
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メタデータID
81003264
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open access
出版タイプ
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タイトル
Cellular Fatty Acid Composition of Stratified Squamous Epithelia After Transplantation of Ex Vivo Produced Oral Mucosa Equivalent
著者
Moriyama, Kentaro ; Yokoo, Satoshi ; Terashi, Hiroto ; Komori, Takahide
著者名
Moriyama, Kentaro
モリヤマ, ケンタロウ
著者名
Yokoo, Satoshi
ヨコオ, サトシ
著者ID
A0056
研究者ID
1000080217421
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=1431d96c128be803520e17560c007669
著者名
Terashi, Hiroto
寺師, 浩人
テラシ, ヒロト
所属機関名
医学部附属病院
著者ID
A0121
研究者ID
1000050251294
著者名
Komori, Takahide
古森, 孝英
コモリ, タカヒデ
所属機関名
医学研究科
収録物名
The Kobe journal of the medical sciences
巻(号)
56(6)
ページ
253-262
出版者
神戸大学医学部
Kobe University School of Medicine
刊行日
2010
公開日
2011-07-21
抄録
Purpose: We have fabricated, for clinical application, artificial oral mucosa that totally excludes both heterogenic protein interaction and xenotransplantation. The purpose of this study is to compare the fatty acid composition of cell membrane phospholipids related to post-transplantation epithelial regeneration. Materials and Methods: Cultured keratinocytes, keratinocytes at 2, 3, 4, and 9 weeks after transplantation, and normal oral keratinocytes were compared by gas chromatography for the composition of 23 fatty acids. The relation between the composition of cell membrane fatty acids, and the glucose metabolism was immunohistochemically analyzed. Results: 1. Even after transplantation, cultured keratinocytes retained the same ratio of palmitic acid as that of normal oral keratinocytes. 2. Essential fatty acids decreased markedly in cultured keratinocyte membranes to the same composition as that of normal oral mucosa 2 weeks after transplantation. 3. The percent composition of palmitoleic acid in cultured keratinocytes was significantly higher than that in post-transplanted keratinocytes; it decreased 2 weeks after artificial mucosa transplantation, but became similar to that in 3 weeks thereafter. 4. The entire population of stratified keratinocytes in EVPOME before transplantation expressed GLUT-1 protein. Conclusion: Our findings suggest that post-artificial mucosa epithelialisation allows keratinocytes to proliferate while consuming palmitic acid, and then diet-provided essential fatty acids induce the keratinocytes to differentiate. Complete clinical epithelialisation of the transplant wound requires 4 weeks; however, within 3 weeks of transplantation, cultured cells of a specific metabolic mechanism change into or are replaced by keratinocytes of a normal metabolic mechanism similar to that of surrounding tissue.
カテゴリ
医学研究科
医学部附属病院
The Kobe journal of the medical sciences
>
56巻
>
56巻6号(2010)
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資源タイプ
departmental bulletin paper
言語
English (英語)
ISSN
0023-2513
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NCID
AA00711740
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URI
http://www.med.kobe-u.ac.jp/journal/contents.html
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