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https://doi.org/10.24546/81012565
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2024-04-26
06:41 集計
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81012565 (fulltext)
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メタデータID
81012565
アクセス権
open access
出版タイプ
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タイトル
Effect of Oral Tributyrin Treatment on Lipid Mediator Profiles in Endotoxin-Induced Hepatic Injury
著者
Miyoshi, Makoto ; Usami, Makoto ; Kajita, Ayumi ; Kai, Motoki ; Nishiyama, Yuya ; Shinohara, Masakazu
著者ID
A1347
研究者ID
1000050433389
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=358199e26bf9770d520e17560c007669
著者名
Miyoshi, Makoto
三好, 真琴
ミヨシ, マコト
所属機関名
保健学研究科
著者名
Usami, Makoto
著者名
Kajita, Ayumi
著者名
Kai, Motoki
著者名
Nishiyama, Yuya
著者ID
A0846
研究者ID
1000080437483
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=7e44708e08ede856520e17560c007669
著者名
Shinohara, Masakazu
篠原, 正和
シノハラ, マサカズ
所属機関名
医学研究科
収録物名
The Kobe journal of the medical sciences
巻(号)
66(4)
ページ
129-138
出版者
神戸大学医学部
Kobe University School of Medicine
刊行日
2020
公開日
2021-03-05
抄録
Eicosanoid modulation by butyrate has been reported in various cells and conditions. Recently, comprehensive analyses of lipid mediators using liquid chromatography/tandem mass spectrometry has been reported. We hypothesized that tributyrin, a prodrug of butyrate, may attenuate LPS-induced liver injury in rats by suppressing the production of pro-inflammatory lipid mediators and/or by inducing anti-inflammatory specialized proresolving mediators. To test this, groups of Wistar rats were orally administered tributyrin (1 g/kg body weight) or vehicle 1 h before intraperitoneal injection of LPS. The livers were collected at 0, 1.5, 6, and 24 h later and analyzed: lipid mediators were profiled by liquid chromatography/tandem mass spectrometry; expression of cyclooxygenase-2, 5-lipoxygenase (LOX), 12/15-LOX, and leukotriene (LT) A4 hydrolase, and nuclear translocation of 5-LOX were evaluated by western blot analysis; and induction of liver injury was assessed by immunostaining for 8-hydroxy-2′-deoxyguanosine, an indicator of oxidative DNA damage. We found that tributyrin treatment attenuated LPS-induced production of pro-inflammatory LTB4 (p < 0.05) and decreased oxidative stress levels in the liver. Tributyrin also attenuated the nuclear translocation of 5-LOX in response to LPS, suggesting a possible mechanism for the LTB4 reduction. LPS-induced changes in other lipid mediators were not significantly affected by tributyrin treatment up to 24 h after LPS injection. Our results suggest that oral tributyrin administration protects against endotoxemia-associated liver damage by reducing production of the pro-inflammatory eicosanoid LTB4.
カテゴリ
医学研究科
保健学研究科
The Kobe journal of the medical sciences
>
66巻
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66巻4号(2020)
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資源タイプ
departmental bulletin paper
言語
English (英語)
ISSN
0023-2513
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NCID
AA00711740
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URI
http://www.med.kobe-u.ac.jp/journal/contents.html
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