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https://hdl.handle.net/20.500.14094/90004189
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2024-04-25
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90004189 (fulltext)
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90004189
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open access
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タイトル
A new-generation of Bacillus subtilis cell factory for further elevated scyllo-inositol production
著者
Tanaka, Kosei ; Natsume, Ayane ; Ishikawa, Shu ; Takenaka, Shinji ; Yoshida, Ken-ichi
著者ID
A1329
研究者ID
1000050418696
著者名
Tanaka, Kosei
田中, 耕生
タナカ, コウセイ
所属機関名
先端融合研究環
著者名
Natsume, Ayane
著者ID
A0019
研究者ID
1000030359872
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=ff864c575b0045a5520e17560c007669
著者名
Ishikawa, Shu
石川, 周
イシカワ, シュウ
所属機関名
科学技術イノベーション研究科
著者ID
A1038
研究者ID
1000040314512
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=c6c1a8027c337e88520e17560c007669
著者名
Takenaka, Shinji
竹中, 慎治
タケナカ, シンジ
所属機関名
農学研究科
著者ID
A1049
研究者ID
1000020230732
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=81199c8c7ea4889b520e17560c007669
著者名
Yoshida, Ken-ichi
吉田, 健一
ヨシダ, ケンイチ
所属機関名
科学技術イノベーション研究科
収録物名
Microbial Cell Factories
巻(号)
16
ページ
67-67
出版者
BioMed Central
刊行日
2017-04-21
公開日
2017-09-22
抄録
Background: A stereoisomer of inositol, scyllo-inositol (SI), has been regarded as a promising therapeutic agent for Alzheimer's disease. However, this compound is relatively rare, whereas another stereoisomer of inositol, myo-inositol (MI) is abundant in nature. Bacillus subtilis 168 has the ability to metabolize inositol stereoisomers, including MI and SI. Previously, we reported a B. subtilis cell factory with modified inositol metabolism that converts MI into SI in the culture medium. The strain was constructed by deleting all genes related to inositol metabolism and overexpressing key enzymes, IolG and IolW. By using this strain, 10 g/l of MI initially included in the medium was completely converted into SI within 48 h of cultivation in a rich medium containing 2% (w/v) Bacto soytone. Results: When the initial concentration of MI was increased to 50 g/l, conversion was limited to 15.1g/l of SI. Therefore, overexpression systems of IolT and PntAB, the main transporter of MI in B. subtilis and the membrane-integral nicotinamide nucleotide transhydrogenase in Escherichia coli respectively, were additionally introduced into the B. subtilis cell factory, but the conversion efficiency hardly improved. We systematically determined the amount of Bacto soytone necessary for ultimate conversion, which was 4% (w/v). As a result, the conversion of SI reached to 27.6 g/l within 48 h of cultivation. Conclusions: The B. subtilis cell factory was improved to yield a SI production rate of 27.6 g/l/48 h by simultaneous overexpression of IolT and PntAB, and by addition of 4% (w/v) Bacto soytone in the conversion medium. The concentration of SI was increased even in the stationary phase perhaps due to nutrients in the Bacto soytone that contribute to the conversion process. Thus, MI conversion to SI may be further optimized via identification and control of these unknown nutrients.
カテゴリ
科学技術イノベーション研究科
先端融合研究環
農学研究科
学術雑誌論文
権利
© The Author(s) 2017.
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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資源タイプ
journal article
言語
English (英語)
eISSN
1475-2859
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関連情報
DOI
https://doi.org/10.1186/s12934-017-0682-0
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