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https://hdl.handle.net/20.500.14094/90004191
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2024-04-20
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90004191 (fulltext)
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メタデータID
90004191
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open access
出版タイプ
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タイトル
An analysis method for flavan-3-ols using high performance liquid chromatography coupled with a fluorescence detector
著者
Wang, Liuqing ; Yamashita, Yoko ; Saito, Akiko ; Ashida, Hitoshi
著者名
Wang, Liuqing
著者ID
A1069
研究者ID
1000010543796
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=035ab271a4f70898520e17560c007669
著者名
Yamashita, Yoko
山下, 陽子
ヤマシタ, ヨウコ
所属機関名
農学研究科
著者名
Saito, Akiko
著者ID
A1023
研究者ID
1000090201889
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=225ad7008aa794ce520e17560c007669
著者名
Ashida, Hitoshi
芦田, 均
アシダ, ヒトシ
所属機関名
農学研究科
収録物名
Journal of Food and Drug Analysis
巻(号)
25(3)
ページ
478-487
出版者
Elsevier Taiwan LLC
刊行日
2017-07
公開日
2017-09-22
抄録
Procyanidins belong to a family of flavan-3-ols, which consist of monomers, (+)-catechin and (-)-epicatechin, and their oligomers and polymers, and are distributed in many plant-derived foods. Procyanidins are reported to have many beneficial physiological activities, such as antihypertensive and anticancer effects. However, the bioavailability of procyanidins is not well understood owing to a lack of convenient and high-sensitive analysis methods. The aim of this study was to develop an improved method for determining procyanidin content in both food materials and biological samples. High performance liquid chromatography (HPLC) coupled with a fluorescence detector was used in this study. The limits of detection (LODs) of (+)-catechin, (-)-epicatechin, procyanidin B2, procyanidin C1, and cinnamtannin A2 were 3.0 x 10(-3) ng, 4.0 x 10(-3) ng, 14.0 x 10(-3) ng, 18.5 x 10(-3) ng, and 23.0 x 10(-3) ng, respectively; the limits of quantification (LOQs) were 10.0 x 10(-3) ng, 29.0 x 10(-3) ng, 28.5 x 10(-3) ng, 54.1 x 10(-3) ng, and 115.0 x 10(-3) ng, respectively. The LOD and LOQ values indicated that the sensitivity of the fluorescence detector method was around 1000 times higher than that of conventional HPLC coupled with a UV-detector. We applied the developed method to measure procyanidins in black soybean seed coat extract (BE) prepared from soybeans grown under three different fertilization conditions, namely, conventional farming, basal manure application, and intertillage. The amount of flavan-3-ols in these BEs decreased in the order intertillage > basal manure application > conventional farming. Commercially available BE was orally administered to mice at a dose of 250 mg/kg body weight, and we measured the blood flavan-3-ol content. Data from plasma analysis indicated that up to the tetramer oligomerization, procyanidins were detectable and flavan-3-ols mainly existed in conjugated forms in the plasma. In conclusion, we developed a highly sensitive and convenient analytical method for the analysis of flavan-3-ols, and applied this technique to investigate the bioavailability of flavan-3-ols in biological samples and to measure flavan-3-ol content in food material and plants.
カテゴリ
農学研究科
学術雑誌論文
権利
© 2017, Food and Drug Administration, Taiwan. Published by Elsevier Taiwan LLC.
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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資源タイプ
journal article
言語
English (英語)
ISSN
1021-9498
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関連情報
DOI
https://doi.org/10.1016/j.jfda.2017.02.005
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