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https://hdl.handle.net/20.500.14094/90004257
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2024-05-08
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90004257 (fulltext)
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1.49 MB
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メタデータID
90004257
アクセス権
open access
出版タイプ
Version of Record
タイトル
Water temperature-dependent degradation of environmental DNA and its relation to bacterial abundance
著者
Tsuji, Satsuki ; Ushio, Masayuki ; Sakurai, Sho ; Minamoto, Toshifumi ; Yamanaka, Hiroki
著者名
Tsuji, Satsuki
著者名
Ushio, Masayuki
著者名
Sakurai, Sho
著者ID
A0574
研究者ID
1000050450656
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=481adea9a93d8196520e17560c007669
著者名
Minamoto, Toshifumi
源, 利文
ミナモト, トシフミ
所属機関名
人間発達環境学研究科
著者名
Yamanaka, Hiroki
収録物名
PLoS ONE
巻(号)
12(4)
ページ
e0176608-e0176608
出版者
Public Library of Science
刊行日
2017-04-27
公開日
2017-10-25
抄録
Environmental DNA (eDNA) is DNA shed by organisms into surrounding environments such as soil and water. The new methods using eDNA as a marker for species detection are being rapidly developed. Here we explore basic knowledge regarding the dependence of the eDNA degradation rate on time and water temperature, and the relationship between eDNA degradation and bacterial abundance. This subject has not been well clarified, even though it is essential for improving the reliability of eDNA analysis. To determine the time-and water temperature-dependent degradation of eDNA, river water was sampled and eDNA concentrations were determined for ayu sweetfish (Plecoglossus altivelis altivelis) and common carp (Cyprinus carpio) at seven time points, over a 48-h period, and at three different water temperatures. The degradation of eDNA was modeled for each species using an existing exponential decay model with an extension to include water temperature effects. The degradation models were constructed for ayu sweetfish as N-t = 229,901.2 x exp [- (0.01062 x k - 0.07081) x t] and for common carp as N-t = 2,558.0 x exp [- (0.01075 x k - 0.07372) x t]. Nt is the DNA concentration at time t (elapsed time in hours) and k is the water temperature (°C). We also measured the concentration of eDNA derived from purified genomic DNA of the common carp, which was spiked into aquarium water without the target species, and we measured the bacterial abundance in the sample water after 12 and 24 h of incubation. Environmental DNA degradation was accelerated at higher water temperatures (generalized linear model, GLM; p < 0.001), but bacterial abundance did not have a significant effect on eDNA degradation (GLM, p = 0.097). These results suggest that the proper treatment of this temperature effect in data interpretations and adjustments would increase the reliability of eDNA analysis in future studies.
カテゴリ
人間発達環境学研究科
学術雑誌論文
権利
© 2017 Tsuji et al.
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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資源タイプ
journal article
言語
English (英語)
eISSN
1932-6203
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関連情報
DOI
https://doi.org/10.1371/journal.pone.0176608
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