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https://hdl.handle.net/20.500.14094/90004258
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2024-04-19
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90004258 (fulltext)
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90004258
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open access
出版タイプ
Version of Record
タイトル
Environmental DNA method for estimating salamander distribution in headwater streams, and a comparison of water sampling methods
著者
Katano, Izumi ; Harada, Ken ; Doi, Hideyuki ; Souma, Rio ; Minamoto, Toshifumi
著者名
Katano, Izumi
著者名
Harada, Ken
著者名
Doi, Hideyuki
著者名
Souma, Rio
著者ID
A0574
研究者ID
1000050450656
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=481adea9a93d8196520e17560c007669
著者名
Minamoto, Toshifumi
源, 利文
ミナモト, トシフミ
所属機関名
人間発達環境学研究科
収録物名
PLoS ONE
巻(号)
12(5)
ページ
e0176541-e0176541
出版者
Public Library of Science
刊行日
2017-05-17
公開日
2017-10-25
抄録
Environmental DNA (eDNA) has recently been used for detecting the distribution of macroor-ganisms in various aquatic habitats. In this study, we applied an eDNA method to estimate the distribution of the Japanese clawed salamander, Onychodactylus japonicus, in headwater streams. Additionally, we compared the detection of eDNA and hand-capturing methods used for determining the distribution of O. japonicus. For eDNA detection, we designed a qPCR primer/probe set for O. japonicus using the 12S rRNA region. We detected the eDNA of O. japonicus at all sites (with the exception of one), where we also observed them by hand-capturing. Additionally, we detected eDNA at two sites where we were unable to observe individuals using the hand-capturing method. Moreover, we found that eDNA concentrations and detection rates of the two water sampling areas (stream surface and under stones) were not significantly different, although the eDNA concentration in the water under stones was more varied than that on the surface. We, therefore, conclude that eDNA methods could be used to determine the distribution of macroorganisms inhabiting headwater systems by using samples collected from the surface of the water.
カテゴリ
人間発達環境学研究科
学術雑誌論文
権利
© 2017 Katano et al.
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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資源タイプ
journal article
言語
English (英語)
eISSN
1932-6203
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関連情報
DOI
https://doi.org/10.1371/journal.pone.0176541
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