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https://hdl.handle.net/20.500.14094/90004360
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2024-04-26
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90004360 (fulltext)
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90004360
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open access
出版タイプ
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タイトル
Engineering a synthetic pathway for maleate in Escherichia coli
著者
Noda, Shuhei ; Shirai, Tomokazu ; Mori, Yutaro ; Oyama, Sachiko ; Kondo, Akihiko
著者名
Noda, Shuhei
著者名
Shirai, Tomokazu
著者名
Mori, Yutaro
著者名
Oyama, Sachiko
著者ID
A1715
研究者ID
1000040205547
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=a324eb4a1b052e53520e17560c007669
著者名
Kondo, Akihiko
近藤, 昭彦
コンドウ, アキヒコ
所属機関名
科学技術イノベーション研究科
収録物名
Nature Communications
巻(号)
8
ページ
1153-1153
出版者
Nature Publishing Group
刊行日
2017-10-27
公開日
2017-11-16
抄録
Maleate is one of the most important dicarboxylic acids and is used to produce various polymer compounds and pharmaceuticals. Herein, microbial production of maleate is successfully achieved, to our knowledge for the first time, using genetically modified Escherichia coli. A synthetic pathway of maleate is constructed in E. coli by combining the polyketide biosynthesis pathway and benzene ring cleavage pathway. The metabolic engineering approach used to fine-tune the synthetic pathway drastically improves maleate production and demonstrates that one of the rate limiting steps exists in the conversion of chorismate to gentisate. In a batch culture of the optimised transformant, grown in a 1-L jar fermentor, the amount of produced maleate reaches 7.1 g L-1, and the yield is 0.221 mol mol(-1). Our results suggest that the construction of synthetic pathways by combining a secondary metabolite pathway and the benzene ring cleavage pathway is a powerful tool for producing various valuable chemicals.
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科学技術イノベーション研究科
学術雑誌論文
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© The Author(s) 2017
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
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資源タイプ
journal article
言語
English (英語)
eISSN
2041-1723
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関連情報
DOI
https://doi.org/10.1038/s41467-017-01233-9
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