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https://hdl.handle.net/20.500.14094/90004363
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2024-04-18
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90004363 (fulltext)
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メタデータID
90004363
アクセス権
open access
出版タイプ
Accepted Manuscript
タイトル
Identifying a breeding habitat of a critically endangered fish, Acheilognathus typus, in a natural river in Japan
著者
Sakata, Masayuki ; Maki, Nobutaka ; Sugiyama, Hideki ; Minamoto, Toshifumi
著者名
Sakata, Masayuki
著者名
Maki, Nobutaka
著者名
Sugiyama, Hideki
著者ID
A0574
研究者ID
1000050450656
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=481adea9a93d8196520e17560c007669
著者名
Minamoto, Toshifumi
源, 利文
ミナモト, トシフミ
所属機関名
人間発達環境学研究科
収録物名
The Science of Nature
巻(号)
104(11-12)
ページ
100-100
出版者
Springer Berlin Heidelberg
刊行日
2017-11-14
公開日
2018-12-03
注記
This is a post-peer-review, pre-copyedit version of an article published in The Science of Nature. The final authenticated version is available online at: https://doi.org/10.1007/s00114-017-1521-1
抄録
Freshwater biodiversity has been severely threatened in recent years, and to conserve endangered species, their distribution and breeding habitats need to be clarified. However, identifying breeding sites in a large area is generally difficult. Here, by combining the emerging environmental DNA (eDNA) analysis with subsequent traditional collection surveys, we successfully identified a breeding habitat for the critically endangered freshwater fish Acheilognathus typus in the mainstream of Omono River in Akita Prefecture, Japan, which is one of the original habitats of this species. Based on DNA cytochrome B sequences of A. typus and closely related species, we developed species-specific primers and a probe that were used in real-time PCR for detecting A. typus eDNA. After verifying the specificity and applicability of the primers and probe on water samples from known artificial habitats, eDNA analysis was applied to water samples collected at 99 sites along Omono River. Two of the samples were positive for A. typus eDNA, and thus, small fixed nets and bottle traps were set out to capture adult fish and verify egg deposition in bivalves (the preferred breeding substrate for A. typus) in the corresponding regions. Mature female and male individuals and bivalves containing laid eggs were collected at one of the eDNA-positive sites. This was the first record of adult A. typus in Omono River in 11 years. This study highlights the value of eDNA analysis to guide conventional monitoring surveys and shows that combining both methods can provide important information on breeding sites that is essential for species’ conservation.
キーワード
Acheilognathus typus
Breeding site
Environmental DNA (eDNA)
Omono River
Real-time PCR
Species-specific detection
カテゴリ
人間発達環境学研究科
学術雑誌論文
権利
The final publication is available at Springer via http://dx.doi.org/10.1007/s00114-017-1521-1
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資源タイプ
journal article
言語
English (英語)
ISSN
0028-1042
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eISSN
1432-1904
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NCID
AA11630527
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関連情報
DOI
https://doi.org/10.1007/s00114-017-1521-1
URI
http://www.kobe-u.ac.jp/research_at_kobe/NEWS/news/2017_11_14_01.html
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