神戸大学附属図書館デジタルアーカイブ
https://hdl.handle.net/20.500.14094/90004566

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メタデータID
90004566
アクセス権
open access
出版タイプ
Accepted Manuscript
タイトル
Comparison of green and albino individuals of the partially mycoheterotrophic orchid Epipactis helleborine on molecular identities of mycorrhizal fungi, nutritional modes and gene expression in mycorrhizal roots
著者
Suetsugu, Kenji ; Yamato, Masahide ; Miura, Chihiro ; Yamaguchi, Katsushi ; Takahashi, Kazuya ; Ida, Yoshiko ; Shigenobu, Shuji ; Kaminaka, Hironori
著者ID
A0447
研究者ID
1000070748839
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=a83853109d6fa20a520e17560c007669
著者名
Suetsugu, Kenji
末次, 健司
スエツグ, ケンジ
所属機関名
理学研究科
著者名
Yamato, Masahide
著者名
Miura, Chihiro
著者名
Yamaguchi, Katsushi
著者名
Takahashi, Kazuya
著者名
Ida, Yoshiko
著者名
Shigenobu, Shuji
著者名
Kaminaka, Hironori
収録物名
Molecular Ecology
巻(号)
26(6)
ページ
1652-1669
出版者
Wiley
刊行日
2017-03
公開日
2018-04-01
抄録
Some green orchids obtain carbon from their mycorrhizal fungi, as well as from photosynthesis. These partially mycoheterotrophic orchids sometimes produce fully achlorophyllous, leaf-bearing (albino) variants. Comparing green and albino individuals of these orchids will help to uncover the molecular mechanisms associated with mycoheterotrophy. We compared green and albino Epipactis helleborine by molecular barcoding of mycorrhizal fungi, nutrient sources based on 15N and 13C abundances, and gene expression in their mycorrhizae by RNA-seq and cDNA de novo assembly. Molecular identification of mycorrhizal fungi showed that green and albino E. helleborine harbored similar mycobionts, mainly Wilcoxina. Stable isotope analyses indicated that albino E. helleborine plants were fully mycoheterotrophic, whereas green individuals were partially mycoheterotrophic. Gene expression analyses showed that genes involved in antioxidant metabolism were up-regulated in the albino variants, which indicates that these plants experience greater oxidative stress than the green variants, possibly due to a more frequent lysis of intracellular pelotons. It was also found that some genes involved in the transport of some metabolites, including carbon sources from plant to fungus, is higher in albino than in green variants. This result may indicate a bidirectional carbon flow even in the mycoheterotrophic symbiosis. The genes related to mycorrhizal symbiosis in autotrophic orchids and arbuscular mycorrhizal plants were also up-regulated in the albino variants, indicating the existence of common molecular mechanisms among the different mycorrhizal types.
キーワード
Epipactis
mixotrophy
mycoheterotrophy
mycorrhizae
partial mycoheterotrophy
RNA sequencing
stable isotope
transcriptome analysis
カテゴリ
理学研究科
学術雑誌論文
権利
© 2017 John Wiley & Sons This is the peer reviewed version of the following article: [Molecular Ecology, 26(6):1652-1669, 2017], which has been published in final form at http://dx.doi.org/10.1111/mec.14021. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.