神戸大学附属図書館デジタルアーカイブ
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https://hdl.handle.net/20.500.14094/90004748
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2024-04-20
16:44 集計
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90004748 (fulltext)
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634 KB
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メタデータID
90004748
アクセス権
open access
出版タイプ
Accepted Manuscript
タイトル
Rapid degradation of longer DNA fragments enables the improved estimation of distribution and biomass using environmental DNA
著者
Jo, Toshiaki ; Murakami, Hiroaki ; Masuda, Reiji ; Sakata, Masayuki K. ; Yamamoto, Satoshi ; Minamoto, Toshifumi
著者名
Jo, Toshiaki
著者名
Murakami, Hiroaki
著者名
Masuda, Reiji
著者名
Sakata, Masayuki K.
著者ID
A0212
研究者ID
1000010643257
著者名
Yamamoto, Satoshi
山本, 哲史
ヤマモト, サトシ
所属機関名
人間発達環境学研究科
著者ID
A0574
研究者ID
1000050450656
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=481adea9a93d8196520e17560c007669
著者名
Minamoto, Toshifumi
源, 利文
ミナモト, トシフミ
所属機関名
人間発達環境学研究科
収録物名
Molecular Ecology Resources
巻(号)
17(6)
ページ
e25-e33
出版者
Wiley
刊行日
2017-11
公開日
2018-12-01
抄録
The advent of environmental DNA (eDNA) analysis methods has enabled rapid and wide-range ecological monitoring in aquatic ecosystems, but there is a dearth of information on eDNA degradation. The results of previous studies suggest that the decay rate of eDNA varies depending on the length of DNA fragments. To examine this hypothesis, we compared temporal change in copy number of long eDNA fragments (719bp) with that of short eDNA fragments (127bp). First, we isolated rearing water from a target fish species, Japanese Jack Mackerel (Trachurus japonicus), and then quantified the copy number of the long and short eDNA fragments in 1L water samples after isolating the water from the fish. Long DNA fragments showed a higher decay rate than short fragments. Next, we measured the eDNA copy numbers of long and short DNA fragments using field samples, and compared them with fish biomass as measured by echo intensity. Although a previous study suggested that short eDNA fragments could be overestimated because of nontarget eDNA from a nearby fish market and carcasses, the eDNA concentrations of long fragments were correlated with echo intensity. This suggests that the concentration of longer eDNA fragments reflects fish biomass more accurately than the previous study by removing the effects of the fish market and carcasses. The length-related differences in eDNA have a substantial potential to improve estimation of species biomass.
キーワード
decay rate
DNA fragment length
echo intensity
environmental DNA (eDNA)
Japanese Jack Mackerel (Trachurus japonicus)
quantitative real-time PCR
カテゴリ
人間発達環境学研究科
学術雑誌論文
権利
© 2017 John Wiley & Sons Ltd. This is the peer reviewed version of the following article: [Molecular Ecology Resources, 17(6):e25-e33, 2017], which has been published in final form at http://dx.doi.org/10.1111/1755-0998.12685. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
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資源タイプ
journal article
言語
English (英語)
ISSN
1755-098X
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eISSN
1755-0998
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関連情報
DOI
https://doi.org/10.1111/1755-0998.12685
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