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https://hdl.handle.net/20.500.14094/90004862
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2024-04-27
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90004862 (fulltext)
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メタデータID
90004862
アクセス権
open access
出版タイプ
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タイトル
Development of a quantitative pachytene chromosome map and its unification with somatic chromosome and linkage maps of rice (Oryza sativa L.)
著者
Ohmido, Nobuko ; Iwata, Aiko ; Kato, Seiji ; Wako, Toshiyuki ; Fukui, Kiichi
著者ID
A0558
研究者ID
1000030343263
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=489af0c45732b381520e17560c007669
著者名
Ohmido, Nobuko
近江戸, 伸子
オオミド, ノブコ
所属機関名
人間発達環境学研究科
著者名
Iwata, Aiko
著者名
Kato, Seiji
著者名
Wako, Toshiyuki
著者名
Fukui, Kiichi
収録物名
PLoS ONE
巻(号)
13(4)
ページ
e0195710-e0195710
出版者
Public Library of Science
刊行日
2018-04-19
公開日
2018-05-16
抄録
A quantitative pachytene chromosome map of rice (Oryza sativa L.) was developed using imaging methods. The map depicts not only distribution patterns of chromomeres specific to pachytene chromosomes, but also the higher order information of chromosomal structures, such as heterochromatin (condensed regions), euchromatin (decondensed regions), the primary constrictions (centromeres), and the secondary constriction (nucleolar organizing regions, NOR). These features were image analyzed and quantitatively mapped onto the map by Chromosome Image Analyzing System ver. 4.0 (CHIAS IV). Correlation between H3K9me2, an epigenetic marker and formation and/or maintenance of heterochromatin, thus was, clearly visualized. Then the pachytene chromosome map was unified with the existing somatic chromosome and linkage maps by physically mapping common DNA markers among them, such as a rice A genome specific tandem repeat sequence (TrsA), 5S and 45S ribosomal RNA genes, five bacterial artificial chromosome (BAC) clones, four P1 bacteriophage artificial chromosome (PAC) clones using multicolor fluorescence in situ hybridization (FISH). Detailed comparison between the locations of the DNA probes on the pachytene chromosomes using multicolor FISH, and the linkage map enabled determination of the chromosome number and short/long arms of individual pachytene chromosomes using the chromosome number and arm assignment designated for the linkage map. As a result, the quantitative pachytene chromosome map was unified with two other major rice chromosome maps representing somatic prometaphase chromosomes and genetic linkages. In conclusion, the unification of the three rice maps serves as an indispensable basic information, not only for an in-depth comparison between genetic and chromosomal data, but also for practical breeding programs.
カテゴリ
人間発達環境学研究科
学術雑誌論文
権利
© 2018 Ohmido et al.
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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資源タイプ
journal article
言語
English (英語)
eISSN
1932-6203
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関連情報
DOI
https://doi.org/10.1371/journal.pone.0195710
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