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https://hdl.handle.net/20.500.14094/90004911
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2024-05-08
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90004911 (fulltext)
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メタデータID
90004911
アクセス権
open access
出版タイプ
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タイトル
Combination therapy with butyrate and docosahexaenoic acid for keloid fibrogenesis: an in vitro study
著者
Torii, Kazuhiro ; Maeshige, Noriaki ; Aoyama-Ishikawa, Michiko ; Miyoshi, Makoto ; Terashi, Hiroto ; Usami, Makoto
著者名
Torii, Kazuhiro
著者ID
A1349
研究者ID
1000090617838
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=bad2e50ccfd61e15520e17560c007669
著者名
Maeshige, Noriaki
前重, 伯壮
マエシゲ, ノリアキ
所属機関名
保健学研究科
著者名
Aoyama-Ishikawa, Michiko
著者ID
A1347
研究者ID
1000050433389
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=358199e26bf9770d520e17560c007669
著者名
Miyoshi, Makoto
三好, 真琴
ミヨシ, マコト
所属機関名
保健学研究科
著者ID
A0056
研究者ID
1000080217421
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=1431d96c128be803520e17560c007669
著者名
Terashi, Hiroto
寺師, 浩人
テラシ, ヒロト
所属機関名
医学部附属病院
著者名
Usami, Makoto
収録物名
Sociedade Brasileira de Dermatologia
巻(号)
92(2)
ページ
184-190
出版者
Soc Brasileira Dermatologia
刊行日
2017-03
公開日
2018-06-04
抄録
Background: A single, effective therapeutic regimen for keloids has not been established yet, and the development of novel therapeutic approaches is expected. Butyrate, a short-chain fatty acid, and docosahexaenoic acid (DHA), a ω-3 polyunsaturated fatty acid, play multiple anti-inflammatory and anticancer roles via their respective mechanisms of action. Objective: In this study, we evaluated the antifibrogenic effects of their single and combined use on keloid fibroblasts. Methods: Keloid fibroblasts were treated with butyrate (0-16 mM) and/or DHA (0-100 μM) for 48 or 96 h. Results: Butyrate inhibited cell proliferation, and α-smooth muscle actin (α-SMA) and type III collagen expressions, with inhibition of the transforming growth factor (TGF)-β1 and TGF-β type I receptor expressions and increased prostaglandin E2 with upregulation of cyclooxygenase-1 expression with induction of histone acetylation. DHA inhibited α-SMA, type III collagen, and TGF-β type I receptor expressions. Then, the butyrate/DHA combination augmented the antifibrogenic effects, resulting in additional inhibition of α-SMA, type I and III collagen expressions, with strong disruption of stress fiber and apoptosis induction. Moreover, the butyrate/DHA combination inhibited the cyclooxygenase-2 expression, suggesting stronger anti-inflammatory effect than each monotherapy. Study limitations: Activation in keloid tissue is affected not only by fibroblasts but also by epithelial cells and immune cells. Evaluation of the effects by butyrate and DHA in these cells or in an in vivo study is required. Conclusion: This study demonstrated that butyrate and docosahexaenoic acid have antifibrogenic effects on keloid fibroblasts and that these may exert therapeutic effects for keloid.
キーワード
Butyrates
Docosahexaenoic acids
Fibroblasts
Fibrosis
Keloid
Prostaglandins E
カテゴリ
医学部附属病院
保健学研究科
学術雑誌論文
権利
©2017 by Anais Brasileiros de Dermatologia.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License which permits unrestricted non-commercial use, distribution, and reproduction in any medium provided the original work is properly cited.
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資源タイプ
journal article
言語
English (英語)
ISSN
0365-0596
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eISSN
1806-4841
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関連情報
DOI
https://doi.org/10.1590/abd1806-4841.20176198
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