神戸大学附属図書館デジタルアーカイブ
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https://hdl.handle.net/20.500.14094/90005120
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2024-04-26
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90005120 (fulltext)
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メタデータID
90005120
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open access
出版タイプ
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タイトル
Development of a modified 3T3 Neutral Red Uptake Phototoxicity Test protocol for evaluation of poorly water-soluble substances
著者
Toyoda, Akemi ; Sugiyama, Maki ; Furihata, Seiichiro ; Nishizumi, Keiji ; Omori, Takashi ; Itagaki, Hiroshi
著者名
Toyoda, Akemi
著者名
Sugiyama, Maki
著者名
Furihata, Seiichiro
著者名
Nishizumi, Keiji
著者ID
A0396
研究者ID
1000050318218
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=3e41a5a210f2b078520e17560c007669
著者名
Omori, Takashi
大森, 崇
オオモリ, タカシ
所属機関名
医学部附属病院
著者名
Itagaki, Hiroshi
収録物名
Journal of Toxicological Sciences
巻(号)
42(5)
ページ
569-577
出版者
The Japanese Society of Toxicology
刊行日
2017-10
公開日
2018-08-21
抄録
The 3T3 neutral red uptake phototoxicity test (OECD TG432) is an alternative phototoxicity test method that is relatively easy and rapid to implement, with results obtainable in a short time, and is reported to have high reproducibility compared with in vivo assay methods. However, this method has been shown to be unsuitable for testing poorly water-soluble substances, which tend to separate out when mixed with the assay buffer solution. This causes difficulties in determining the dose dependency of substances and subsequent determination of the photoirritation factor because the ratio of cell viability, expressed as the half-maximal inhibitory concentration (IC50) in the presence or absence of light, is not calculable. In this study, we investigated the optimum conditions for the evaluation of poorly water-soluble substances. In the conventional method, the final solvent concentration was 1% and the pre-incubation time was 60 min, but in the modified method, 10% and 5 min were used, respectively. Next, the results from the conventional method were compared with those of our modified method, which was found to be viable and comparable with the conventional method. Moreover, the false positive results frequently obtained with poorly water-soluble substances in the conventional method were not evident with the modified method, thus confirming its usefulness for the evaluation of such substances. We therefore propose that the modified method can be used for the in vitro testing of poorly water-soluble substances in phototoxicity evaluations.
キーワード
3T3 NRU PT
Phototoxicity
Photoirritation
Poorly water-soluble
カテゴリ
医学部附属病院
学術雑誌論文
権利
© 2017 The Japanese Society of Toxicology
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資源タイプ
journal article
言語
English (英語)
ISSN
0388-1350
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eISSN
1880-3989
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関連情報
DOI
https://doi.org/10.2131/jts.42.569
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