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https://hdl.handle.net/20.500.14094/90005123
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2024-04-25
17:28 集計
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90005123 (fulltext)
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90005123
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open access
出版タイプ
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タイトル
Estimating the protein burden limit of yeast cells by measuring the expression limits of glycolytic proteins
著者
Eguchi, Yuichi ; Makanae, Koji ; Hasunuma, Tomohisa ; Ishibashi, Yuko ; Kito, Keiji ; Moriya, Hisao
著者名
Eguchi, Yuichi
著者名
Makanae, Koji
著者ID
A0960
研究者ID
1000020529606
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=73b63639d47d0a4b520e17560c007669
著者名
Hasunuma, Tomohisa
蓮沼, 誠久
ハスヌマ, トモヒサ
所属機関名
先端バイオ工学研究センター
著者名
Ishibashi, Yuko
著者名
Kito, Keiji
著者名
Moriya, Hisao
収録物名
eLife
巻(号)
7
ページ
e34595-e34595
出版者
eLife Sciences Publications
刊行日
2018-08-10
公開日
2018-08-22
抄録
The ultimate overexpression of a protein could cause growth defects, which are known as the protein burden. However, the expression limit at which the protein-burden effect is triggered is still unclear. To estimate this limit, we systematically measured the overexpression limits of glycolytic proteins in Saccharomyces cerevisiae. The limits of some glycolytic proteins were up to 15% of the total cellular protein. These limits were independent of the proteins' catalytic activities, a finding that was supported by an in silico analysis. Some proteins had low expression limits that were explained by their localization and metabolic perturbations. The codon usage should be highly optimized to trigger the protein-burden effect, even under strong transcriptional induction. The S-S-bond-connected aggregation mediated by the cysteine residues of a protein might affect its expression limit. Theoretically, only non-harmful proteins could be expressed up to the protein-burden limit. Therefore, we established a framework to distinguish proteins that are harmful and non-harmful upon overexpression.
カテゴリ
先端バイオ工学研究センター
学術雑誌論文
権利
© 2018 Eguchi et al.
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
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資源タイプ
journal article
言語
English (英語)
eISSN
2050-084X
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DOI
https://doi.org/10.7554/eLife.34595.001
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