神戸大学附属図書館デジタルアーカイブ
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学内刊行物
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https://hdl.handle.net/20.500.14094/90005215
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2024-04-20
23:49 集計
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90005215 (fulltext)
pdf
5.42 MB
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メタデータID
90005215
アクセス権
open access
出版タイプ
Accepted Manuscript
タイトル
Structural differences between myofibrillar protein, paratropomyosin, and tropomyosin as revealed by high-performance liquid chromatography
著者
Nishio, Yuriko ; Ushimura, Yuichi ; Ueda, Shuji ; Maeda, Naoyuki ; Hattori, Akihito ; Yamanoue, Minoru
著者名
Nishio, Yuriko
著者名
Ushimura, Yuichi
著者ID
A1058
研究者ID
1000050379400
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=06b23bdf963d8d6a520e17560c007669
著者名
Ueda, Shuji
上田, 修司
ウエダ, シュウジ
所属機関名
農学研究科
著者名
Maeda, Naoyuki
著者名
Hattori, Akihito
著者ID
A1022
研究者ID
1000030182596
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=355a31e42cc28d47520e17560c007669
著者名
Yamanoue, Minoru
山之上, 稔
ヤマノウエ, ミノル
所属機関名
農学研究科
収録物名
Animal Science Journal
巻(号)
89(8)
ページ
1161-1168
出版者
Wiley
刊行日
2018-08
公開日
2019-09-01
抄録
Paratropomyosin (PTM) composes myofibril functions to weaken the rigor linkages formed between actin and myosin during postmortem aging of muscles. PTM has the similar physico-chemical properties as tropomyosin ™ that is a regulatory protein of myofibrils. So far, it is unclear whether PTM is definitely different from TM, because the primary structure of PTM has not been determined yet. The aim of this study was to clarify structural difference of PTM from TM. PTM was prepared by column chromatography immediately after slaughter from broiler breast muscle, and purified by high-performance liquid chromatography (HPLC). Purified PTM was successfully separated from TM, and the recovered PTM molecule was reduced with dithiothreitol to separate again by HPLC. Two subunits were obtained and peptides from each digested subunit by V8 protease were recovered by HPLC, and then amino acid sequences of the peptides were analyzed by protein sequencing. As a result, some amino acid residues were replaced from that of TMα1 isoform which is the major isoform of TM, and also was different between the two subunits. Therefore, it is concluded that PTM clearly differs from TM and it is suggested that functional difference in PTM from TM is attributed to amino acid replacements in subunits composing PTM.
キーワード
HPLC
meat tenderness
myofibrillar protein
paratropomyosin
カテゴリ
農学研究科
学術雑誌論文
権利
© 2018 Japanese Society of Animal Science. This is the peer reviewed version of the following article: [Animal Science Journal, 89(8):1161-1168, 2018], which has been published in final form at https://doi.org/10.1111/asj.13022. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
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資源タイプ
journal article
言語
English (英語)
ISSN
1344-3941
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eISSN
1740-0929
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関連情報
DOI
https://doi.org/10.1111/asj.13022
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