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https://hdl.handle.net/20.500.14094/90005251
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2024-04-23
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90005251 (fulltext)
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メタデータID
90005251
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open access
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タイトル
Development of a strictly regulated xylose-induced expression system in Streptomyces
著者
Noguchi, Yuji ; Kashiwagi, Norimasa ; Uzura, Atsuko ; Ogino, Chiaki ; Kondo, Akihiko ; Ikeda, Haruo ; Sota, Masahiro
著者名
Noguchi, Yuji
著者ID
A0177
研究者ID
1000080587387
著者名
Kashiwagi, Norimasa
柏木, 紀賢
カシワギ, ノリマサ
所属機関名
科学技術イノベーション研究科
著者名
Uzura, Atsuko
著者ID
A0339
研究者ID
1000000313693
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=cb7c84b4e252c367520e17560c007669
著者名
Ogino, Chiaki
荻野, 千秋
オギノ, チアキ
所属機関名
工学研究科
著者ID
A1715
研究者ID
1000040205547
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=a324eb4a1b052e53520e17560c007669
著者名
Kondo, Akihiko
近藤, 昭彦
コンドウ, アキヒコ
所属機関名
科学技術イノベーション研究科
著者名
Ikeda, Haruo
著者名
Sota, Masahiro
収録物名
Microbial Cell Factories
巻(号)
17
ページ
151-151
出版者
BMC
刊行日
2018-09-21
公開日
2018-10-05
抄録
Background: Genetic tools including constitutive and inducible promoters have been developed over the last few decades for strain engineering in Streptomyces. Inducible promoters are useful for controlling gene expression, however only a limited number are applicable to Streptomyces. The aim of this study is to develop a controllable protein expression system based on an inducible promoter using sugar inducer, which has not yet been widely applied in Streptomyces. Results: To determine a candidate promoter, inducible protein expression was first examined in Streptomyces avermitilis MA-4680 using various carbon sources. Xylose isomerase (xylA) promoter derived from xylose (xyl) operon was selected due to strong expression of xylose isomerase (XylA) in the presence of D-xylose. Next, a xylose-inducible protein expression system was constructed by investigating heterologous protein expression (chitobiase as a model protein) driven by the xylA promoter in Streptomyces lividans. Chitobiase activity was detected at high levels in S. lividans strain harboring an expression vector with xylA promoter (pXC), under both xylose-induced and non-induced conditions. Thus, S. avermitilis xylR gene, which encodes a putative repressor of xyl operon, was introduced into constructed vectors in order to control protein expression by D-xylose. Among strains constructed in the study, XCPR strain harboring pXCPR vector exhibited strict regulation of protein expression. Chitobiase activity in the XCPR strain was observed to be 24 times higher under xylose-induced conditions than that under non-induced conditions. Conclusion: In this study, a strictly regulated protein expression system was developed based on a xylose-induced system. As far as we could ascertain, this is the first report of engineered inducible protein expression in Streptomyces by means of a xylose-induced system. This system might be applicable for controllable expression of toxic products or in the field of synthetic biology using Streptomyces strains.
キーワード
Xylose
Xylose isomerase promoter
Streptomyces avermitilis
Streptomyces lividans
Heterologous protein production
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科学技術イノベーション研究科
工学研究科
学術雑誌論文
権利
© The Author(s) 2018.
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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資源タイプ
journal article
言語
English (英語)
eISSN
1475-2859
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DOI
https://doi.org/10.1186/s12934-018-0991-y
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