神戸大学附属図書館デジタルアーカイブ
入力補助
English
カテゴリ
学内刊行物
ランキング
アクセスランキング
ダウンロードランキング
https://hdl.handle.net/20.500.14094/90005345
このアイテムのアクセス数:
22
件
(
2024-04-24
03:28 集計
)
閲覧可能ファイル
ファイル
フォーマット
サイズ
閲覧回数
説明
90005345 (fulltext)
pdf
3.12 MB
6
メタデータ
ファイル出力
メタデータID
90005345
アクセス権
open access
出版タイプ
Version of Record
タイトル
Bradyrhizobium diazoefficiens USDA110 PhaR functions for pleiotropic regulation of cellular processes besides PHB accumulation
著者
Nishihata, Shogo ; Kondo, Takahiko ; Tanaka, Kosei ; Ishikawa, Shu ; Takenaka, Shinji ; Kang, Choong-Min ; Yoshida, Ken-ichi
著者名
Nishihata, Shogo
著者名
Kondo, Takahiko
著者ID
A1329
研究者ID
1000050418696
著者名
Tanaka, Kosei
田中, 耕生
タナカ, コウセイ
所属機関名
先端融合研究環
著者ID
A0019
研究者ID
1000030359872
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=ff864c575b0045a5520e17560c007669
著者名
Ishikawa, Shu
石川, 周
イシカワ, シュウ
所属機関名
科学技術イノベーション研究科
著者ID
A1038
研究者ID
1000040314512
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=c6c1a8027c337e88520e17560c007669
著者名
Takenaka, Shinji
竹中, 慎治
タケナカ, シンジ
所属機関名
農学研究科
著者名
Kang, Choong-Min
著者ID
A1049
研究者ID
1000020230732
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=81199c8c7ea4889b520e17560c007669
著者名
Yoshida, Ken-ichi
吉田, 健一
ヨシダ, ケンイチ
所属機関名
科学技術イノベーション研究科
収録物名
BMC Microbiology
巻(号)
18
ページ
156-156
出版者
BMC
刊行日
2018-10-24
公開日
2018-11-16
抄録
Background Bradyrhizobium diazoefficiens USDA110 nodulates soybeans for nitrogen fixation. It accumulates poly-3-hydroxybutyrate (PHB), which is of physiological importance as a carbon/energy source for survival during starvation, infection, and nitrogen fixation conditions. PHB accumulation is orchestrated by not only the enzymes for PHB synthesis but also PHB-binding phasin proteins (PhaPs) stabilizing the PHB granules. The transcription factor PhaR controls the phaP genes. Results Inactivation of phaR led to decreases in PHB accumulation, less cell yield, increases in exopolysaccharide (EPS) production, some improvement in heat stress tolerance, and slightly better growth under microaerobic conditions. Changes in the transcriptome upon phaR inactivation were analyzed. PhaR appeared to be involved in the repression of various target genes, including some PHB-degrading enzymes and others involved in EPS production. Furthermore, in vitro gel shift analysis demonstrated that PhaR bound to the promoter regions of representative targets. For the phaP1 and phaP4 promoter regions, PhaR-binding sites were determined by DNase I footprinting, allowing us to deduce a consensus sequence for PhaR-binding as TGCRNYGCASMA (R: A or G, Y: C or T, S: C or G, M: A or C). We searched for additional genes associated with a PhaR-binding sequence and found that some genes involved in central carbon metabolism, such as pdhA for pyruvate dehydrogenase and pckA for phosphoenolpyruvate carboxykinase, may be regulated positively and directly by PhaR. Conclusions These results suggest that PhaR could regulate various genes not only negatively but also positively to coordinate metabolism holistically in response to PHB accumulation.
キーワード
Bradyrhizobium diazoefficiens
Poly-3-hydroxybutyrate (PHB)
Transcriptome
Transcription factor
DNA binding
カテゴリ
科学技術イノベーション研究科
先端融合研究環
農学研究科
学術雑誌論文
権利
© The Author(s). 2018.
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
詳細を表示
資源タイプ
journal article
言語
English (英語)
eISSN
1471-2180
OPACで所蔵を検索
CiNiiで学外所蔵を検索
関連情報
DOI
https://doi.org/10.1186/s12866-018-1317-2
ホームへ戻る