神戸大学附属図書館デジタルアーカイブ
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https://hdl.handle.net/20.500.14094/90005399
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2024-04-27
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90005399 (fulltext)
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メタデータID
90005399
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open access
出版タイプ
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タイトル
Enhancing 3-hydroxypropionic acid production in combination with sugar supply engineering by cell surface-display and metabolic engineering of Schizosaccharomyces pombe
著者
Takayama, Seiya ; Ozaki, Aiko ; Konishi, Rie ; Otomo, Chisako ; Kishida, Mayumi ; Hirata, Yuuki ; Matsumoto, Takuya ; Tanaka, Tsutomu ; Kondo, Akihiko
著者名
Takayama, Seiya
著者名
Ozaki, Aiko
著者名
Konishi, Rie
著者名
Otomo, Chisako
著者名
Kishida, Mayumi
著者名
Hirata, Yuuki
著者ID
A1342
研究者ID
1000040727161
著者名
Matsumoto, Takuya
松本, 拓也
マツモト, タクヤ
所属機関名
科学技術イノベーション研究科
著者ID
A0311
研究者ID
1000090436551
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=106238a6648c3bb7520e17560c007669
著者名
Tanaka, Tsutomu
田中, 勉
タナカ, ツトム
所属機関名
工学研究科
著者ID
A1715
研究者ID
1000040205547
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=a324eb4a1b052e53520e17560c007669
著者名
Kondo, Akihiko
近藤, 昭彦
コンドウ, アキヒコ
所属機関名
科学技術イノベーション研究科
収録物名
Microbial Cell Factories
巻(号)
17
ページ
176-176
出版者
BMC
刊行日
2018-11-13
公開日
2018-11-28
抄録
Background Economical production of value-added chemicals from renewable biomass is a promising path to sustainability. 3-Hydroxypropionic acid (3-HP) is an important chemical for building a bio-sustainable society. Establishment of 3-HP production from renewable resources such as glucose would provide a bio-sustainable alternative to the production of acrylic acid from fossil resources. Results Here, we describe metabolic engineering of the fission yeast Schizosaccharomyces pombe to enhance 3-HP production from glucose and cellobiose via the malonyl-CoA pathway. The mcr gene, encoding the malonyl-CoA reductase of Chloroflexus aurantiacus, was dissected into two functionally distinct fragments, and the activities of the encoded protein were balanced. To increase the cellular supply of malonyl-CoA and acetyl-CoA, we introduced genes encoding endogenous aldehyde dehydrogenase, acetyl-CoA synthase from Salmonella enterica, and endogenous pantothenate kinase. The resulting strain produced 3-HP at 1.0g/L from a culture starting at a glucose concentration of 50g/L. We also engineered the sugar supply by displaying beta-glucosidase (BGL) on the yeast cell surface. When grown on 50g/L cellobiose, the beta-glucosidase-displaying strain consumed cellobiose efficiently and produced 3-HP at 3.5g/L. Under fed-batch conditions starting from cellobiose, this strain produced 3-HP at up to 11.4g/L, corresponding to a yield of 11.2% (g-3-HP/g-glucose; given that 1g cellobiose corresponds to 1.1g glucose upon digestion). Conclusions In this study, we constructed a series of S. pombe strains that produced 3-HP via the malonyl-CoA pathway. Our study also demonstrated that BGL display using cellobiose and/or cello-oligosaccharides as a carbon source has the potential to improve the titer and yield of malonyl-CoA- and acetyl-CoA-derived compounds.
キーワード
Schizosaccharomyces pombe
3-Hydroxypropionic acid
CRISPR-Cas9 system
Cell surface display
Malonyl-CoA
カテゴリ
科学技術イノベーション研究科
工学研究科
学術雑誌論文
権利
© The Author(s) 2018.
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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資源タイプ
journal article
言語
English (英語)
eISSN
1475-2859
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関連情報
DOI
https://doi.org/10.1186/s12934-018-1025-5
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