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https://hdl.handle.net/20.500.14094/90005469
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2024-05-02
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90005469 (fulltext)
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メタデータID
90005469
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open access
出版タイプ
Version of Record
タイトル
Endosymbiosis-related changes in ultrastructure and chemical composition of Chlorella variabilis (Archaeplastida, Chlorophyta) cell wall in Paramecium bursaria (Ciliophora, Oligohymcnophorea)
著者
Higuchi, Rina ; Song, Chihong ; Hoshina, Ryo ; Suzaki, Toshinobu
著者名
Higuchi, Rina
著者名
Song, Chihong
著者名
Hoshina, Ryo
著者ID
A0004
研究者ID
1000000187692
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=092cc814fa1a4846520e17560c007669
著者名
Suzaki, Toshinobu
洲﨑, 敏伸
スザキ, トシノブ
所属機関名
理学研究科
収録物名
European Journal of Protistology
巻(号)
66
ページ
149-155
出版者
Elsevier GmbH
刊行日
2018-10
公開日
2018-12-20
抄録
Chlorella variabilis, a symbiotic alga, is usually present in the cytoplasm of Paramecium bursaria, although it can be cultured in host-free conditions. Morphological and chemical properties of its cell wall were compared between its free-living and symbiotic states. Transmission electron microscopy (quick-freezing and freeze-substitution methods) revealed that the cell wall thickness of symbiotic C. variabilis was reduced to about half that of the free-living one. Chemical properties of the cell wall were examined by treatment with three fluorescent reagents (calcofluor white M2R, FITC-WGA, and FITC-LFA) having specific binding affinities to different polysaccharides. When the algae were re-introduced into Paramecium host cells, calcofluor fluorescence intensity reduced by about 50%. Calcofluor stains β-d-glucopyranose polysaccharides such as cellulose, N-acetylglucosamine, sialic acid, and glycosaminoglycans. Because treatment with cellulase showed no effect on calcofluor fluorescence intensity, we consider that cellulose is not majorly responsible for the stainability of calcofluor. Staining intensities of FITC-WGA and FITC-LFA were similar in the free-living and symbiotic conditions, suggesting that N-acetylglucosamine and sialic acid are also not responsible for the reduction in the stainability of calcofluor associated with intracellular symbiosis. The amount of glycosaminoglycans on the cell wall may decrease in C. variabilis present in the cytoplasm of P. bursaria.
キーワード
Cell wall
Chlorella variabilis
Glycosaminoglycans
Paramecium bursaria
Symbiosis
カテゴリ
理学研究科
学術雑誌論文
権利
© 2018 The Authors. Published by Elsevier GmbH.
This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
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資源タイプ
journal article
言語
English (英語)
ISSN
0932-4739
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eISSN
1618-0429
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関連情報
DOI
https://doi.org/10.1016/j.ejop.2018.09.003
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