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https://hdl.handle.net/20.500.14094/90005570
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2024-03-29
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90005570 (fulltext)
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メタデータID
90005570
アクセス権
open access
出版タイプ
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タイトル
Genome-wide characterization of DNA methylation, small RNA expression, and histone H3 lysine nine di-methylation in Brassica rapa L.
著者
Takahashi, Satoshi ; Osabe, Kenji ; Fukushima, Naoki ; Takuno, Shohei ; Miyaji, Naomi ; Shimizu, Motoki ; Takasaki-Yasuda, Takeshi ; Suzuki, Yutaka ; Dennis, Elizabeth S. ; Seki, Motoaki ; Fujimoto, Ryo
著者名
Takahashi, Satoshi
著者名
Osabe, Kenji
著者名
Fukushima, Naoki
著者名
Takuno, Shohei
著者名
Miyaji, Naomi
著者名
Shimizu, Motoki
著者ID
A1036
研究者ID
1000030314511
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/profile/ja.fceab13135b002fa520e17560c007669.html
著者名
Takasaki-Yasuda, Takeshi
安田, 剛志
ヤスダ, タケシ
所属機関名
農学研究科
著者名
Suzuki, Yutaka
著者名
Dennis, Elizabeth S.
著者名
Seki, Motoaki
著者ID
A0337
研究者ID
1000060620375
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/profile/ja.2d794652078d165f520e17560c007669.html
著者名
Fujimoto, Ryo
藤本, 龍
フジモト, リョウ
所属機関名
農学研究科
収録物名
DNA Research
巻(号)
25(5)
ページ
511-520
出版者
Oxford University Press (OUP)
刊行日
2018-10-01
公開日
2019-02-06
抄録
Epigenetic gene regulation is crucial to plant life and can involve dynamic interactions between various histone modifications, DNA methylation, and small RNAs. Detailed analysis of epigenome information is anticipated to reveal how the DNA sequence of the genome is translated into the plant's phenotype. The aim of this study was to map the DNA methylation state at the whole genome level and to clarify the relationship between DNA methylation and transcription, small RNA expression, and histone H3 lysine 9 di-methylation (H3K9me2) in Brassica rapa. We performed whole genome bisulfite sequencing, small RNA sequencing, and chromatin immunoprecipitation sequencing using H3K9me2 antibody in a Chinese cabbage inbred line, RJKB-T24, and examined the impact of epigenetic states on transcription. Cytosine methylation in DNA was analysed in different sequence contexts (CG, CHG, and CHH) (where H could be A, C, or T) and position (promoter, exon, intron, terminator, interspersed repeat regions), and the H3K9me2 and 24 nucleotide small interfering RNAs (24nt-siRNA) were overlaid onto the B. rapa reference genome. The epigenome was compared with that of Arabidopsis thaliana and the relationship between the position of DNA methylation and gene expression, and the involvement of 24 nt siRNAs and H3K9me2 are discussed.
キーワード
DNA methylation
histone H3 lysine 9 di-methylation
small RNA
epigenetic
Brassica
カテゴリ
農学研究科
学術雑誌論文
権利
© The Author(s) 2018. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
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資源タイプ
journal article
言語
English (英語)
ISSN
1340-2838
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eISSN
1756-1663
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関連情報
DOI
https://doi.org/10.1093/dnares/dsy021
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