神戸大学附属図書館デジタルアーカイブ
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https://hdl.handle.net/20.500.14094/90005601
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2024-04-20
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90005601 (fulltext)
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メタデータID
90005601
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open access
出版タイプ
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タイトル
Genetic variants of Helicobacter pylori type IV secretion system components CagL and CagI and their association with clinical outcomes
著者
Ogawa, Hirofumi ; Iwamoto, Akira ; Tanahashi, Toshihito ; Okada, Rina ; Yamamoto, Koji ; Nishiumi, Shin ; Yoshida, Masaru ; Azuma, Takeshi
著者名
Ogawa, Hirofumi
著者名
Iwamoto, Akira
著者名
Tanahashi, Toshihito
著者名
Okada, Rina
著者名
Yamamoto, Koji
著者ID
A0815
研究者ID
1000020514706
著者名
Nishiumi, Shin
西海, 信
ニシウミ, シン
所属機関名
医学研究科
著者ID
A0791
研究者ID
1000000419475
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=b42c4a25405e79a1520e17560c007669
著者名
Yoshida, Masaru
吉田, 優
ヨシダ, マサル
所属機関名
医学研究科
著者ID
A0058
研究者ID
1000060221040
著者名
Azuma, Takeshi
東, 健
アズマ, タケシ
所属機関名
医学研究科
収録物名
Gut Pathogens
巻(号)
9
ページ
21-21
出版者
BMC (part of Springer Nature)
刊行日
2017-04-21
公開日
2019-02-19
抄録
Background:Helicobacter pylori infection is associated with risk for chronic gastritis (CG), gastric ulcer (GU), duodenal ulcer (DU), and gastric cancer (GC). The H. pylori Cag type IV secretion system (TFSS) translocates the virulence factor cytotoxin-associated gene A protein into host cells and plays an important role in initiating gastric carcinogenesis. The CagL and CagI proteins are components of the TFSS. The Arg-Gly-Asp (RGD) motif of CagL, and the six most distal C-terminal amino acids (Ser-Lys-Ile-Ile-Val-Lys, and Ser-Lys-Val-Ile-Val-Lys) of CagL and CagI are essential for TFSS adhe-sion to host cells. Additionally, the CagL variant Tyr58Glu59 was previously shown to be associated with GC patients.Results: We isolated 43 H. pylori isolates from 17 CG, 8 GU, 8 DU, and 10 GC patients in Southeast Asia. Total DNAs were extracted and sequenced with MiSeq. H. pylori strain ATCC 26695, which was isolated from CG patients, was used as a reference. We examined the full sequences of H. pylori cagL and cagI using whole-genome sequencing (WGS), and analyzed whether single nucleotide variants and amino acid changes (AACs) correlated with adverse clinical outcomes. Three isolates were excluded from the analysis due to cagPAI rearrangements. CagL RGD motifs were conserved in 39 isolates (97.5%). CagL-Glu59 and Ile234 in the C-terminal motif were more common in 10 H. pyloriisolates from GC patients (p < 0.001 and p < 0.05, respectively). When 5 Vietnamese isolates from GC patients were excluded, CagL-Glu59 still remains significant (p < 0.05), but not Ile234. CagL-Tyr58 was seen in only one isolate. The CagI C-terminal motif was completely conserved across all 40 isolates, and there were no significant AACs in CagI.Conclusions: Using WGS, we analyzed genetic variants in clinical H. pylori isolates and identified putative novel and candidate variants in uncharacterized CagL and CagI sequences that are related to gastric carcinogenesis. In particu-lar, CagL-Glu59 has the possible association with GC.
キーワード
Helicobacter pylori
Whole-genome sequencing
Type IV secretion system
CagL
CagI
カテゴリ
医学研究科
学術雑誌論文
権利
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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資源タイプ
journal article
言語
English (英語)
eISSN
1757-4749
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関連情報
DOI
https://doi.org/10.1186/s13099-017-0165-1
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