神戸大学附属図書館デジタルアーカイブ
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学内刊行物
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https://hdl.handle.net/20.500.14094/90005608
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2024-05-09
02:14 集計
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90005608 (fulltext)
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690 KB
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メタデータID
90005608
アクセス権
open access
出版タイプ
Accepted Manuscript
タイトル
Nanofluidic Biosensor Created by Bonding Patterned Model Cell Membrane and Silicone Elastomer with Silica Nanoparticles
著者
Tanabe, Masashi ; Ando, Koji ; Komatsu, Ryota ; Morigaki, Kenichi
著者名
Tanabe, Masashi
著者名
Ando, Koji
著者名
Komatsu, Ryota
著者ID
A1236
研究者ID
1000010358179
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=f6c039bc884596f8520e17560c007669
著者名
Morigaki, Kenichi
森垣, 憲一
モリガキ, ケンイチ
所属機関名
バイオシグナル総合研究センター
収録物名
Small
巻(号)
14(49)
ページ
1802804-1802804
出版者
Wiley‐VCH Verlag
刊行日
2018-12-06
公開日
2020-01-01
抄録
Selective and sensitive detection of specific molecules in a solution containing diverse coexisting molecules is important in many biomedical and environmental applications, including diagnostics and pollutant detection. Here, a nanofluidic biosensor is developed to detect specific target molecules (e.g., toxin proteins) in the presence of nontarget molecules by bonding a patterned model cell membrane and a silicone elastomer (polydimethylsiloxane: PDMS) sheet using surface-modified silica nanoparticles as the adhesive layer. Owing to the uniform size of nanoparticles, a nanometric gap junction is formed between the fluid bilayer and PDMS (nanogap-junction). The thickness of the nanogap-junction is controlled by the size of the silica nanoparticles. Target molecules that specifically bind to the receptor molecules in the fluid bilayer are selectively transported into the nanogap-junction via lateral diffusion through the lipid membrane. A thinner gap formed with smaller nanoparticles can enhance the sensitivity (signal-to-background ratio) more effectively, owing to the suppression of nonspecific penetration of coexisting molecules. Silica nanoparticles also provide excellent mechanical robustness, realizing long-term stability of the gap structure. Nanogap-junction using silica nanoparticles provides a versatile platform for highly selective and sensitive sensing by realizing detection of specific target molecules in a solution containing more concentrated nontarget molecules.
キーワード
biosensor
gap-junction
lipid bilayer
nanofluidics
silica nanoparticle
カテゴリ
バイオシグナル総合研究センター
学術雑誌論文
権利
© 2018 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is the accepted version of the following article: [Small, 14(49):1802804, 2018], which has been published in final form at https://doi.org/10.1002/smll.201802804. This article may be used for non-commercial purposes in accordance with the Wiley Self-Archiving Policy
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資源タイプ
journal article
言語
English (英語)
ISSN
1613-6810
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eISSN
1613-6829
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NCID
AA11975212
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関連情報
DOI
https://doi.org/10.1002/smll.201802804
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