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https://hdl.handle.net/20.500.14094/90005635
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2024-04-25
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90005635 (fulltext)
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メタデータID
90005635
アクセス権
open access
出版タイプ
Version of Record
タイトル
Fabrication of a Novel Culture Dish Adapter with a Small Recess Structure for Flow Control in a Closed Environment
著者
Yasuda, Reiko ; Adachi, Shungo ; Okonogi, Atsuhito ; Anzai, Youhei ; Kamiyama, Tadataka ; Katano, Keiji ; Hoshi, Nobuhiko ; Natsume, Tohru ; Mogi, Katsuo
著者名
Yasuda, Reiko
著者名
Adachi, Shungo
著者名
Okonogi, Atsuhito
著者名
Anzai, Youhei
著者名
Kamiyama, Tadataka
著者名
Katano, Keiji
著者ID
A0039
研究者ID
1000010209223
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=fa741cc09ab44f05520e17560c007669
著者名
Hoshi, Nobuhiko
星, 信彦
ホシ, ノブヒコ
所属機関名
先端融合研究環
著者名
Natsume, Tohru
著者名
Mogi, Katsuo
収録物名
Applied Sciences
巻(号)
9(2)
ページ
269-269
出版者
MDPI
刊行日
2019-01-14
公開日
2019-03-06
抄録
Cell culture medium replacement is necessary to replenish nutrients and remove waste products, and perfusion and batch media exchange methods are available. The former can establish an environment similar to that in vivo, and microfluidic devices are frequently used. However, these methods are hampered by incompatibility with commercially available circular culture dishes and the difficulty in controlling liquid flow. Here, we fabricated a culture dish adapter using polydimethylsiloxane that has a small recess structure for flow control compatible with commercially available culture dishes. We designed U-shaped and I-shaped recess structure adapters and we examined the effects of groove structure on medium flow using simulation. We found that the U-shaped and I-shaped structures allowed a uniform and uneven flow of medium, respectively. We then applied these adaptors to 293T cell culture and examined the effects of recess structures on cell proliferation. As expected, cell proliferation was similar in each area of a dish in the U-shaped structure adapter, whereas in the early flow area in the I-shaped structure adapter, it was significantly higher. In summary, we succeeded in controlling liquid flow in culture dishes with the fabricated adapter, as well as in applying the modulation of culture medium flow to control cell culture.
キーワード
flow control
culture dish adapter
small recess structure
closed environment
perfusion culture
カテゴリ
先端融合研究環
学術雑誌論文
権利
© 2019 by the authors. Licensee MDPI, Basel, Switzerland.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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資源タイプ
journal article
言語
English (英語)
eISSN
2076-3417
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関連情報
DOI
https://doi.org/10.3390/app9020269
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