神戸大学附属図書館デジタルアーカイブ
入力補助
English
カテゴリ
学内刊行物
ランキング
アクセスランキング
ダウンロードランキング
https://hdl.handle.net/20.500.14094/90005659
このアイテムのアクセス数:
52
件
(
2024-04-27
05:46 集計
)
閲覧可能ファイル
ファイル
フォーマット
サイズ
閲覧回数
説明
90005659 (fulltext)
pdf
2.66 MB
2
メタデータ
ファイル出力
メタデータID
90005659
アクセス権
open access
出版タイプ
Version of Record
タイトル
Quercetin and its metabolite isorhamnetin promote glucose uptake through different signalling pathways in myotubes
著者
Jiang, Hao ; Yamashita, Yoko ; Nakamura, Asuka ; Croft, Kevin ; Ashida, Hitoshi
著者名
Jiang, Hao
著者ID
A1069
研究者ID
1000010543796
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=035ab271a4f70898520e17560c007669
著者名
Yamashita, Yoko
山下, 陽子
ヤマシタ, ヨウコ
所属機関名
農学研究科
著者名
Nakamura, Asuka
著者名
Croft, Kevin
著者ID
A1023
研究者ID
1000090201889
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=225ad7008aa794ce520e17560c007669
著者名
Ashida, Hitoshi
芦田, 均
アシダ, ヒトシ
所属機関名
農学研究科
収録物名
Scientific Reports
巻(号)
9
ページ
2690-2690
出版者
Nature Research
刊行日
2019-02-25
公開日
2019-03-11
抄録
Quercetin and its metabolite isorhamnetin elicit various beneficial effects on human health. However, their bioavailability is low. In this study, we investigated whether low concentrations in the physiological range could promote glucose uptake in L6 myotubes, as well as the underlying molecular mechanisms. We found that 0.1 nM and 1 nM quercetin or 1 nM isorhamnetin significantly increased glucose uptake via translocation of glucose transporter type 4 (GLUT4) to the plasma membrane of L6 myotubes. Quercetin principally activated the CaMKK beta/AMPK signalling pathway at these concentrations, but also activated IRS1/PI3K/Akt signalling at 10 nM. In contrast, 1 nM and 10 nM isorhamnetin principally activated the JAK/STAT pathway. Treatment with siAMPK alpha and siJAK2 abolished quercetin- and isorhamnetin-induced GLUT4 translocation, respectively. However, treatment with siJAK3 did not affect isorhamnetin-induced GLUT4 translocation, indicating that isorhamnetin induced GLUT4 translocation mainly through JAK2, but not JAK3, signalling. Thus, quercetin preferably activated the AMPK pathway and, accordingly, stimulated IRS1/PI3K/Akt signalling, while isorhamnetin activated the JAK2/STAT pathway. Furthermore, after oral administration of quercetin glycoside at 10 and 100 mg/kg body weight significantly induced GLUT4 translocation to the plasma membrane of skeletal muscles in mice. In the same animals, plasma concentrations of quercetin aglycone form were 4.95 and 6.80 nM, respectively. In conclusion, at low-concentration ranges, quercetin and isorhamnetin promote glucose uptake by increasing GLUT4 translocation via different signalling pathways in skeletal muscle cells; thus, these compounds may possess beneficial functions for maintaining glucose homeostasis by preventing hyperglycaemia at physiological concentrations.
カテゴリ
農学研究科
学術雑誌論文
権利
© The Author(s) 2019.
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
詳細を表示
資源タイプ
journal article
言語
English (英語)
eISSN
2045-2322
OPACで所蔵を検索
CiNiiで学外所蔵を検索
関連情報
DOI
https://doi.org/10.1038/s41598-019-38711-7
ホームへ戻る