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https://hdl.handle.net/20.500.14094/90005773
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2024-04-26
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90005773 (fulltext)
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90005773
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open access
出版タイプ
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タイトル
Structural insight into microtubule stabilization and kinesin inhibition by Tau family MAPs
著者
Shigematsu, Hideki ; Imasaki, Tsuyoshi ; Doki, Chihiro ; Sumi, Takuya ; Aoki, Mari ; Uchikubo-Kamo,Tomomi ; Sakamoto, Ayako ; Tokuraku, Kiyotaka ; Shirouzu, Mikako ; Nitta, Ryo
著者名
Shigematsu, Hideki
著者ID
A2313
研究者ID
1000060631661
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=9838ec4cfdfa0741520e17560c007669
著者名
Imasaki, Tsuyoshi
今崎, 剛
イマサキ, ツヨシ
所属機関名
医学研究科
著者名
Doki, Chihiro
著者名
Sumi, Takuya
著者名
Aoki, Mari
著者名
Uchikubo-Kamo,Tomomi
著者名
Sakamoto, Ayako
著者名
Tokuraku, Kiyotaka
著者名
Shirouzu, Mikako
著者名
Nitta, Ryo
収録物名
Journal of Cell Biology
巻(号)
217(12)
ページ
4155-4163
出版者
Rockefeller University Press
刊行日
2018-10-01
公開日
2019-04-01
抄録
The Tau family microtubule-associated proteins (MAPs) promote microtubule stabilization and regulate microtubule-based motility. They share the C-terminal microtubule-binding domain, which includes three to five tubulin-binding repeats. Different numbers of repeats formed by alternative splicing have distinct effects on the activities of these proteins, and the distribution of these variants regulates fundamental physiological phenomena in cells. In this study, using cryo-EM, we visualized the MAP4 microtubule complex with the molecular motor kinesin-1. MAP4 bound to the C-terminal domains of tubulins along the protofilaments stabilizes the longitudinal contacts of the microtubule. The strongest bond of MAP4 was found around the intertubulin–dimer interface such that MAP4 coexists on the microtubule with kinesin-1 bound to the intratubulin–dimer interface as well. MAP4, consisting of five repeats, further folds and accumulates above the intertubulin–dimer interface, interfering with kinesin-1 movement. Therefore, these cryo-EM studies reveal new insight into the structural basis of microtubule stabilization and inhibition of kinesin motility by the Tau family MAPs.
カテゴリ
医学研究科
学術雑誌論文
権利
© 2018 Shigematsu et al.http://www.rupress.org/terms/
This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
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資源タイプ
journal article
言語
English (英語)
ISSN
0021-9525
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eISSN
1540-8140
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関連情報
DOI
https://doi.org/10.1083/jcb.201711182
URI
http://www.kobe-u.ac.jp/research_at_kobe/NEWS/news/2018_10_02_01.html
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