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https://hdl.handle.net/20.500.14094/90005798
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2024-04-26
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90005798 (fulltext)
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メタデータID
90005798
アクセス権
open access
出版タイプ
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タイトル
Environmental DNA reveals nonmigratory individuals of Palaemon paucidens overwintering in Lake Biwa shallow waters
著者
Wu, Qianqian ; Kawano, Ken ; Uehara, Yoshitoshi ; Okuda, Noboru ; Hongo, Masamichi ; Tsuji, Satsuki ; Yamanaka, Hiroki ; Minamoto, Toshifumi
著者名
Wu, Qianqian
著者名
Kawano, Ken
著者名
Uehara, Yoshitoshi
著者名
Okuda, Noboru
著者名
Hongo, Masamichi
著者名
Tsuji, Satsuki
著者名
Yamanaka, Hiroki
著者ID
A0574
研究者ID
1000050450656
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=481adea9a93d8196520e17560c007669
著者名
Minamoto, Toshifumi
源, 利文
ミナモト, トシフミ
所属機関名
人間発達環境学研究科
収録物名
Freshwater Science
巻(号)
37(2)
ページ
307-314
出版者
University of Chicago Press
刊行日
2018-06
公開日
2019-04-08
抄録
In Lake Biwa, the largest freshwater lake in Japan, the lacustrine shrimp Palaemon paucidens has been reported to undertake seasonal migrations, living in deep waters from autumn to winter and in shallow waters from spring to summer. Some investigators have suggested that some individuals overwinter in shallow waters, but the ecology and life history of such nonmigratory populations are poorly understood. We developed a species-specific environmental DNA (eDNA) marker to detect P. paucidens in water samples and used it to examine the distribution and relative abundance of overwintering individuals in the shallow waters of Lake Biwa. Water samples were collected from 21 shore sites and 32 surrounding freshwater lagoons of Lake Biwa in November 2015 and February 2016. In November, 4 shore and 12 freshwater lagoon sites were positive for P. paucidens eDNA, and in February, 4 shore and 9 freshwater lagoon sites were positive. The relative abundance of eDNA copies was estimated by quantitative real-time polymerase chain reaction (PCR). Simultaneous sampling of P. paucidens and its eDNA, conducted at the inlet and outlet of 5 freshwater lagoons in December 2016, validated our method as a quantitative measure of the relative abundance of P. paucidens local populations. The eDNA approach used here confirmed that some P. paucidens individuals overwinter in the shallow waters, suggesting life-history diversity in Lake Biwa populations.
キーワード
environmental DNA
eDNA
Palaemon paucidens
migration
Lake Biwa
カテゴリ
人間発達環境学研究科
学術雑誌論文
権利
© 2018 by The Society for Freshwater Science.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0), which permits non-commercial reuse of the work with attribution. For commercial use, contact journalpermissions@press.uchicago.edu.
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資源タイプ
journal article
言語
English (英語)
ISSN
2161-9549
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eISSN
2161-9565
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関連情報
DOI
https://doi.org/10.1086/697542
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