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https://hdl.handle.net/20.500.14094/90005813
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2024-04-25
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90005813 (fulltext)
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メタデータID
90005813
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open access
出版タイプ
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タイトル
Site-specific post-imprinting modification of molecularly imprinted polymer nanocavities with a modifiable functional monomer for prostate cancer biomarker recognition
著者
Matsumoto, Hiroki ; Sunayama, Hirobumi ; Kitayama, Yukiya ; Takano, Eri ; Takeuchi, Toshifumi
著者名
Matsumoto, Hiroki
著者名
Sunayama, Hirobumi
著者ID
A0981
研究者ID
1000040649745
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=e0d918d3a04b2f62520e17560c007669
著者名
Kitayama, Yukiya
北山, 雄己哉
キタヤマ, ユキヤ
所属機関名
工学研究科
著者ID
A0144
研究者ID
1000020634645
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=a01e5ff359cc4e9a520e17560c007669
著者名
Takano, Eri
高野, 恵里
タカノ, エリ
所属機関名
工学研究科
著者ID
A1283
研究者ID
1000070179612
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=c0fc57bdfc2ff8a5520e17560c007669
著者名
Takeuchi, Toshifumi
竹内, 俊文
タケウチ, トシフミ
所属機関名
工学研究科
収録物名
Science and Technology of Advanced Materials
巻(号)
20(1)
ページ
305-312
出版者
Taylor & Francis Ltd
刊行日
2019-01-01
公開日
2019-04-11
抄録
Recognition of glycans of glycoproteins using biotic materials such as antibodies is challenging due to lack of antigenicity. Polymeric materials suitable for the molecular recognition of glycoproteins have attracted considerable attention. In this study, we aimed to develop abiotic molecular materials for the recognition of prostate-specific antigen (PSA), a known biomarker for prostate cancer. We used a non-covalent bonding-based molecular imprinting technique to introduce post-imprinting poly(ethylene glycol)-based capping agent into a low-affinity recognition cavity. Details of the binding properties of these groups were investigated to optimize their affinity and selectivity for PSA. Molecularly imprinted polymers (MIPs) were prepared using a bottom-up approach based on surface-initiated atom transfer radical polymerization from a PSA-conjugated sensor chip with a functional monomer-bearing carboxy and secondary amine groups as interaction and post-imprinting modification (PIM) sites, respectively. PSA was orientationally conjugated on the sensor chip through diesters between the immobilized 3-fluorophenyl boronic acid and the cis-diol groups of PSA glucans. Treatment with the capping agent selectively inactivated low-affinity recognition cavities while protecting high-affinity cavities with the addition of a low concentration of PSA as a dynamic protection agent. The MIP thickness is critical in the present molecular imprinting, as a value of less than 5 nm can enable high selectivity. We believe that the proposed strategy based on a non-covalent molecular imprinting approach combined with a PIM-based capping treatment provides a novel method for the development of highly sensitive and selective glycoprotein recognition materials for use in biomarker sensing.
キーワード
Molecular imprinting
post-imprinting modifications
biomarker sensing
カテゴリ
工学研究科
学術雑誌論文
権利
© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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資源タイプ
journal article
言語
English (英語)
ISSN
1468-6996
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eISSN
1878-5514
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関連情報
DOI
https://doi.org/10.1080/14686996.2019.1583495
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