神戸大学附属図書館デジタルアーカイブ
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https://hdl.handle.net/20.500.14094/90005874
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2024-04-20
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90005874 (fulltext)
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1.48 MB
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メタデータID
90005874
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open access
出版タイプ
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タイトル
Less biomass and intracellular glutamate in anodic biofilms lead to efficient electricity generation by microbial fuel cells
著者
Sasaki, Daisuke ; Sasaki, Kengo ; Tsuge, Yota ; Kondo, Akihiko
著者ID
A0276
研究者ID
1000000650615
著者名
Sasaki, Daisuke
佐々木, 大介
ササキ, ダイスケ
所属機関名
科学技術イノベーション研究科
著者ID
A0278
研究者ID
1000050558301
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=543788ae48fc1b54520e17560c007669
著者名
Sasaki, Kengo
佐々木, 建吾
ササキ, ケンゴ
所属機関名
科学技術イノベーション研究科
著者名
Tsuge, Yota
著者ID
A1715
研究者ID
1000040205547
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=a324eb4a1b052e53520e17560c007669
著者名
Kondo, Akihiko
近藤, 昭彦
コンドウ, アキヒコ
所属機関名
科学技術イノベーション研究科
収録物名
Biotechnology for Biofuels
巻(号)
12
ページ
72-72
出版者
BMC
刊行日
2019-04-01
公開日
2019-04-22
抄録
Background Using a microbial fuel cell (MFC), we observed that a complex microbial community decomposed starch and transferred electrons to a graphite felt anode to generate current. In spite of the same reactor configuration, inoculum, substrate, temperature, and pH, MFCs produced different current and power density. To understand which factor(s) affected electricity generation, here, we analyzed a complex microbial community in an anodic biofilm and fermentation broth using Illumina MiSeq sequencing and metabolomics. Results Microbial biomass on the anode was lower in MFCs generating more electricity (0.09–0.16 mg cm−2-anode) than in those generating less electricity (0.60–2.80 mg cm(-2)-anode), while being equal (3890–4196 mg L(−1)-broth) in the fermentation broth over the same operational period. Chemical oxygen demand removal and acetate concentration were also similar in fermentation broths. MFCs generating more electricity had relatively more exoelectrogenic bacteria, such as Geobacter sp., but fewer acetate-utilizing Methanosarcina sp. and/or Lactococcus sp. in anodic biofilms. Accordingly, anodic biofilms generating more electricity presented higher levels of most intracellular metabolites related to the tricarboxylic acid cycle and a higher intracellular ATP/ADP ratio, but a lower intracellular NADH/NAD(+) ratio. Moreover, the level of intracellular glutamate, an essential metabolite for microbial anabolic reactions, correlated negatively with current density. Conclusion Microbial growth on the anode and intracellular glutamate levels negatively affect electricity generation by MFCs. Reduced formation of anodic biofilm, in which intracellular glutamate concentration is 33.9 μmol g-cell(−1) or less, favors the growth of acetate-utilizing Geobacter sp. on the anode and improves current generation.
キーワード
Microbial fuel cell
Microbial community
Anodic biofilm
Biomass
Geobacter
Intracellular glutamate
カテゴリ
科学技術イノベーション研究科
学術雑誌論文
権利
© The Author(s) 2019.
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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資源タイプ
journal article
言語
English (英語)
eISSN
1754-6834
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関連情報
DOI
https://doi.org/10.1186/s13068-019-1414-y
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