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学内刊行物
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https://hdl.handle.net/20.500.14094/90006048
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2024-04-27
04:33 集計
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90006048 (fulltext)
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586 KB
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メタデータID
90006048
アクセス権
open access
出版タイプ
Accepted Manuscript
タイトル
Application of next-generation sequencing to the study of non-model insects
著者
Wachi, Nakatada ; Matsubayashi, Kei W. ; Maeto, Kaoru
著者名
Wachi, Nakatada
著者名
Matsubayashi, Kei W.
著者ID
A1046
研究者ID
1000080346238
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=8b48aa3c6c1af65e520e17560c007669
著者名
Maeto, Kaoru
前藤, 薫
マエトウ, カオル
所属機関名
農学研究科
収録物名
Entomological Science
巻(号)
21(1)
ページ
3-11
出版者
Wiley
刊行日
2018-03
公開日
2019-05-31
抄録
Recent developments in, and widespread availability of, platforms, technologies, and computer software and hardware have enhanced the use of genetic markers to address major scientific questions in non-model organisms, even by researchers with limited expertise in genomics. However, there are few studies investigating the genomes of non-model insects using these approaches. This review discusses the application of next-generation sequencing (NGS) technologies to the study of genomes of wild organisms. We first introduce currently available NGS-based methods-including restriction site-associated DNA sequencing; multiplexed inter-simple sequence repeat genotyping by sequencing; target capture; and amplicon, transcriptome, and whole genome sequencing-as useful tools for studies of non-model insects. We also provide guidelines for first-time users of NGS systems. In addition to the massive amount of information that it provides, a major advantage of NGS data is the scalability to future research projects. Studies using NGS technology can answer questions related to basic entomology by focusing on the unique nature of non-model insects in wild environments in a way that is not possible for model organisms.
キーワード
amplicon sequencing
high-throughput sequencing
MIG-seq
non-model organism
RAD-seq
RNA-seq
target capture
カテゴリ
農学研究科
学術雑誌論文
権利
© 2017 The Entomological Society of Japan. This is the peer reviewed version of the following article: [Entomological Science, 21(1):3-11, 2018], which has been published in final form at https://doi.org/10.1111/ens.12281. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
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資源タイプ
journal article
言語
English (英語)
ISSN
1343-8786
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eISSN
1479-8298
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関連情報
DOI
https://doi.org/10.1111/ens.12281
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