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https://hdl.handle.net/20.500.14094/90006421
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2024-04-25
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90006421 (fulltext)
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メタデータID
90006421
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open access
出版タイプ
Version of Record
タイトル
Day/Night Separation of Oxygenic Energy Metabolism and Nuclear DNA Replication in the Unicellular Red Alga Cyanidioschyzon merolae
著者
Miyagishima, Shin-ya ; Era, Atsuko ; Hasunuma, Tomohisa ; Matsuda, Mami ; Hirooka, Shunsuke ; Sumiya, Nobuko ; Kondo, Akihiko ; Fujiwara, Takayuki
著者名
Miyagishima, Shin-ya
著者名
Era, Atsuko
著者ID
A0960
研究者ID
1000020529606
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=73b63639d47d0a4b520e17560c007669
著者名
Hasunuma, Tomohisa
蓮沼, 誠久
ハスヌマ, トモヒサ
所属機関名
先端バイオ工学研究センター
著者名
Matsuda, Mami
著者名
Hirooka, Shunsuke
著者名
Sumiya, Nobuko
著者ID
A1715
研究者ID
1000040205547
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=a324eb4a1b052e53520e17560c007669
著者名
Kondo, Akihiko
近藤, 昭彦
コンドウ, アキヒコ
所属機関名
科学技術イノベーション研究科
著者名
Fujiwara, Takayuki
収録物名
mBio
巻(号)
10(4)
ページ
e00833-19
出版者
American Society for Microbiology
刊行日
2019-07
公開日
2019-10-21
抄録
The transition from G(1) to S phase and subsequent nuclear DNA replication in the cells of many species of eukaryotic algae occur predominantly during the evening and night in the absence of photosynthesis; however, little is known about how day/night changes in energy metabolism and cell cycle progression are coordinated and about the advantage conferred by the restriction of S phase to the night. Using a synchronous culture of the unicellular red alga Cyanidioschyzon merolae, we found that the levels of photosynthetic and respiratory activities peak during the morning and then decrease toward the evening and night, whereas the pathways for anaerobic consumption of pyruvate, produced by glycolysis, are upregulated during the evening and night as reported recently in the green alga Chlamydomonas reinhardtii. Inhibition of photosynthesis by 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) largely reduced respiratory activity and the amplitude of the day/night rhythm of respiration, suggesting that the respiratory rhythm depends largely on photosynthetic activity. Even when the timing of G(1)/S-phase transition was uncoupled from the day/night rhythm by depletion of retinoblastoma-related (RBR) protein, the same patterns of photosynthesis and respiration were observed, suggesting that cell cycle progression and energy metabolism are regulated independently. Progression of the S phase under conditions of photosynthesis elevated the frequency of nuclear DNA double-strand breaks (DSB). These results suggest that the temporal separation of oxygenic energy metabolism, which causes oxidative stress, from nuclear DNA replication reduces the risk of DSB during cell proliferation in C. merolae.
キーワード
cell cycle
Cyanidioschyzon merolae
endosymbiosis
photosynthetic oxidative stress
カテゴリ
科学技術イノベーション研究科
先端バイオ工学研究センター
学術雑誌論文
権利
© 2019 Miyagishima et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
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資源タイプ
journal article
言語
English (英語)
eISSN
2150-7511
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関連情報
DOI
https://doi.org/10.1128/mBio.00833-19
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