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https://hdl.handle.net/20.500.14094/90006560
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2024-03-30
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90006560 (fulltext)
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メタデータID
90006560
アクセス権
open access
出版タイプ
Version of Record
タイトル
Fat-specific protein 27α inhibits autophagy-dependent lipid droplet breakdown in white adipocytes
著者
Nakajima, Shinsuke ; Nishimoto, Yuki ; Tateya, Sanshiro ; Iwahashi, Yasuyuki ; Okamatsu-Ogura, Yuko ; Saito, Masayuki ; Ogawa, Wataru ; Tamori, Yoshikazu
著者名
Nakajima, Shinsuke
著者名
Nishimoto, Yuki
著者名
Tateya, Sanshiro
著者名
Iwahashi, Yasuyuki
著者名
Okamatsu-Ogura, Yuko
著者名
Saito, Masayuki
著者ID
A1358
研究者ID
1000040294219
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/profile/ja.38228987a3a438c8520e17560c007669.html
著者名
Ogawa, Wataru
小川, 渉
オガワ, ワタル
所属機関名
医学研究科
著者ID
A1938
研究者ID
1000090379397
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/profile/ja.fc3ce686271f4d14520e17560c007669.html
著者名
Tamori, Yoshikazu
田守, 義和
タモリ, ヨシカズ
所属機関名
医学研究科
収録物名
Journal of Diabetes Investigation
巻(号)
10(6)
ページ
1419-1429
出版者
Wiley
刊行日
2019-11
公開日
2019-12-06
抄録
Aims/Introduction Fat-specific protein 27 (FSP27)α is the major isoform of FSP27 in white adipose tissue (WAT), and is essential for large unilocular lipid droplet (LD) formation in white adipocytes. In contrast, FSP27β is abundantly expressed in brown adipose tissue (BAT), and plays an important role in small multilocular LD formation. In FSP27 KO mice in which FSP27α and β are both depleted, WAT is characterized by multilocular LD formation, and by increased mitochondrial abundance and energy expenditure, whereas BAT conversely manifests large oligolocular LDs and reduced energy expenditure. Materials and Methods We investigated the effects of autophagy in WAT and BAT of wild type (WT) and FSP27 knockout (KO) mice. In addition, we examined the effects of FSP27α and FSP27β to the induction of autophagy in COS cells. Results Food deprivation induced autophagy in BAT of WT mice, as well as in WAT of FSP27 KO mice, suggesting that enhanced autophagy is characteristic of adipocytes with small multilocular LDs. Pharmacological inhibition of autophagy attenuated the fasting-induced loss of LD area in adipocytes with small multilocular LDs (BAT of WT mice and WAT of FSP27 KO mice), without affecting that in adipocytes with large unilocular or oligolocular LDs (WAT of WT mice or in BAT of FSP27 KO mice). Overexpression of FSP27α inhibited autophagy induction by serum deprivation in COS cells, whereas that of FSP27β had no such effect. Conclusions The present results thus showed that FSP27α inhibits autophagy and might thereby contribute to the energy-storage function of WAT.
キーワード
Autophagy
Fat-specific protein 27
White adipocytes
カテゴリ
医学研究科
学術雑誌論文
権利
© 2019 The Authors. Journal of Diabetes Investigation published by Asian Association for the Study of Diabetes (AASD) and John Wiley & Sons Australia, Ltd
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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資源タイプ
journal article
言語
English (英語)
ISSN
2040-1116
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eISSN
2040-1124
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NCID
AA12488319
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関連情報
DOI
https://doi.org/10.1111/jdi.13050
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