神戸大学附属図書館デジタルアーカイブ
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学内刊行物
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https://hdl.handle.net/20.500.14094/90006607
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2024-04-24
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90006607 (fulltext)
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メタデータID
90006607
アクセス権
open access
出版タイプ
Accepted Manuscript
タイトル
Self-Spreading of Phospholipid Bilayer in a Patterned Framework of Polymeric Bilayer
著者
Tamura, Fuyuko ; Tanimoto, Yasushi ; Nagai, Rurika ; Hayashi, Fumio ; Morigaki, Kenichi
著者名
Tamura, Fuyuko
著者名
Tanimoto, Yasushi
著者名
Nagai, Rurika
著者名
Hayashi, Fumio
著者ID
A1236
研究者ID
1000010358179
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=f6c039bc884596f8520e17560c007669
著者名
Morigaki, Kenichi
森垣, 憲一
モリガキ, ケンイチ
所属機関名
バイオシグナル総合研究センター
収録物名
Langmuir
巻(号)
35(45)
ページ
14696-14703
出版者
American Chemical Society
刊行日
2019-11-12
公開日
2020-11-01
注記
Published online 15 October 2019
抄録
Phospholipid bilayers spontaneously spread on a hydrophilic substrate such as glass in aqueous solution due to the energetic gain of surface wetting. This process (self-spreading) was utilized to form a patterned model biological membrane containing reconstituted membrane proteins. A mechanically stable framework of a polymerized lipid bilayer was first generated by the lithographic polymerization of a diacetylene phospholipid. Then, natural lipid membranes (fluid bilayers) were introduced into the channels between polymeric bilayers by the self-spreading from a phospholipid reservoir. The spreading velocity could be fitted into a slope of −0.5 in a double logarithmic plot versus time due to the balance between the spreading force and resistive drag. The preformed polymeric bilayer accelerated the spreading by the energetic gain of covering hydrophobic edges with a lipid bilayer. At the same time, the domains of the polymeric bilayer obstructed spreading, and the spreading velocity linearly decreased with their fractional coverage. Above the critical coverage of ca. 50%, self-spreading was completely blocked (percolation threshold) and the fluid bilayer was confined in the polymer-free regions. Nonspecific adsorption of lipids onto the surface of polymeric bilayers was negligible, which enabled a heightened signal-to-background ratio in the reconstitution and observation of membrane proteins. Self-spread bilayers had a higher density of lipids than those formed by the spontaneous rupture of vesicles (vesicle fusion), presumably due to the continual supply of lipid molecules from the reservoir. These features give the self-spreading important advantages for preparing patterned model membranes with reconstituted membrane proteins.
カテゴリ
バイオシグナル総合研究センター
学術雑誌論文
権利
This document is the Accepted Manuscript version of a Published Work that appeared in final form in Langmuir, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.langmuir.9b02685
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資源タイプ
journal article
言語
English (英語)
ISSN
0743-7463
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eISSN
1520-5827
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NCID
AA10461730
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関連情報
DOI
https://doi.org/10.1021/acs.langmuir.9b02685
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