神戸大学附属図書館デジタルアーカイブ
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https://hdl.handle.net/20.500.14094/90006784
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2024-04-18
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90006784 (fulltext)
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メタデータID
90006784
アクセス権
open access
出版タイプ
Version of Record
タイトル
Hepatic Artery Embolization Induces the Local Overexpression of Transforming Growth Factor β1 in a Rat Hepatoma Model
著者
Ueshima, Eisuke ; Nishiofuku, Hideyuki ; Takaki, Haruyuki ; Hirata, Yutaka ; Kodama, Hiroshi ; Tanaka, Toshihiro ; Kichikawa, Kimihiko ; Yamakado, Koichiro ; Okada, Takuya ; Sofue, Keitaro ; Yamaguchi, Masato ; Sugimoto, Koji ; Murakami, Takamichi
著者ID
A1505
研究者ID
1000040645561
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=27f5782f098b3c4a520e17560c007669
著者名
Ueshima, Eisuke
上嶋, 英介
ウエシマ, エイスケ
所属機関名
医学部附属病院
著者名
Nishiofuku, Hideyuki
著者名
Takaki, Haruyuki
著者名
Hirata, Yutaka
著者名
Kodama, Hiroshi
著者名
Tanaka, Toshihiro
著者名
Kichikawa, Kimihiko
著者名
Yamakado, Koichiro
著者ID
A1460
研究者ID
1000080514786
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=1b7a24e9e768e586520e17560c007669
著者名
Okada, Takuya
岡田, 卓也
オカダ, タクヤ
所属機関名
医学部附属病院
著者ID
A1469
研究者ID
1000090622027
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=f7fcd4a60d373990520e17560c007669
著者名
Sofue, Keitaro
祖父江, 慶太郎
ソフエ, ケイタロウ
所属機関名
医学部附属病院
著者ID
A1392
研究者ID
1000010457096
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=24afca231ae41ee9520e17560c007669
著者名
Yamaguchi, Masato
山口, 雅人
ヤマグチ, マサト
所属機関名
医学部附属病院
著者ID
A0768
研究者ID
1000090314476
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=558b017116277cc8520e17560c007669
著者名
Sugimoto, Koji
杉本, 幸司
スギモト, コウジ
所属機関名
医学部附属病院
著者ID
A2302
研究者ID
1000020252653
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=4d5c2d2510f6d3a2520e17560c007669
著者名
Murakami, Takamichi
村上, 卓道
ムラカミ, タカミチ
所属機関名
医学研究科
収録物名
Liver Cancer
巻(号)
9(1)
ページ
63-72
出版者
Karger
刊行日
2020-02
公開日
2020-02-20
抄録
Introduction: The underlying mechanism involved in the recurrence of hepatoma after hepatic arterial embolization (HAE) is not adequately examined. An immunosuppressive cytokine, transforming growth factor β1 (TGF-β1), can lead to tumor progression and is affected by hypoxia in various cancers. The study aimed to assess the effect of HAE on the expression of TGF-β1 in a rat hepatoma model. Methods: Sprague-Dawley rats bearing N1S1 hepatoma cells underwent HAE (HAE group, n = 5) or sham treatment (sham group, n = 4). The animals were euthanized at 48 h, and liver tissues were harvested. Immunohistochemistry (IHC) and quantitative polymerase chain reaction (qPCR) were performed to compare the expression of TGF-β1 and hypoxia-inducible factor 1α (HIF-1α) between the HAE and sham groups. In vitro experiments with the N1S1 cell line were also performed under normoxic (21% O2) or hypoxic (1% O2) conditions for 48 h, and the expression of TGF-β1 and HIF-1α was assessed with western blotting and enzyme-linked immunosorbent assay. Statistical data comparisons were performed by Student t test. Results: IHC showed that both the TGF-β1-positive and HIF-1α-positive tumor peripheral areas were larger in the HAE group (6.59 ± 2.49 and 10.26 ± 4.14%; p < 0.001, respectively) than in the sham group (0.34 ± 0.41 and 0.40 ± 0.84% respectively). Similarly, qPCR showed that the mRNA expression levels of TGF-β1 and HIF-1α were higher (1.95 ± 0.38-fold and 1.62 ± 0.37-fold; p < 0.001 and p = 0.002, respectively) in the HAE group than those in the sham group. TGF-β1 expression was suppressed when HIF-1α inhibitors were added (p = 0.001), and HIF-1α expression was upregulated when exogenous TGF-β1 was added (p = 0.033) in N1S1 cells. Conclusion: HAE enhanced local TGF-β1 expression in a rat hepatoma model. In vitro experiments suggest that HAE-induced hypoxic stress may trigger the interdependent expression of TGF-β1 and HIF-1α.
キーワード
Hepatic arterial embolization
Hepatoma
Hypoxia-inducible factor 1α
Hypoxia
Transforming growth factor β1
カテゴリ
医学研究科
医学部附属病院
学術雑誌論文
権利
© 2019 The Author(s) Published by S. Karger AG, Basel. This article is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND) (http://www.karger.com/Services/OpenAccessLicense). Usage and distribution for commercial purposes as well as any distribution of modified material requires written permission.
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資源タイプ
journal article
言語
English (英語)
ISSN
2235-1795
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eISSN
1664-5553
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NCID
AA12610897
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関連情報
DOI
https://doi.org/10.1159/000502774
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