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https://hdl.handle.net/20.500.14094/90006977
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2024-04-25
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90006977 (fulltext)
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90006977
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open access
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A bacterial cell factory converting glucose into scyllo-inositol, a therapeutic agent for Alzheimer's disease
著者
Michon, Christophe ; Kang, Choong-Min ; Karpenko, Sophia ; Tanaka, Kosei ; Ishikawa, Shu ; Yoshida, Ken-ichi
著者名
Michon, Christophe
著者名
Kang, Choong-Min
著者名
Karpenko, Sophia
著者ID
A1329
研究者ID
1000050418696
著者名
Tanaka, Kosei
田中, 耕生
タナカ, コウセイ
所属機関名
先端融合研究環
著者ID
A0019
研究者ID
1000030359872
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=ff864c575b0045a5520e17560c007669
著者名
Ishikawa, Shu
石川, 周
イシカワ, シュウ
所属機関名
科学技術イノベーション研究科
著者ID
A1049
研究者ID
1000020230732
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=81199c8c7ea4889b520e17560c007669
著者名
Yoshida, Ken-ichi
吉田, 健一
ヨシダ, ケンイチ
所属機関名
科学技術イノベーション研究科
収録物名
Communications Biology
巻(号)
3(1)
ページ
93-93
出版者
Nature Research
刊行日
2020-03-02
公開日
2020-03-30
抄録
A rare stereoisomer of inositol, scyllo-inositol, is a therapeutic agent that has shown potential efficacy in preventing Alzheimer's disease. Mycobacterium tuberculosis ino1 encoding myo-inositol-1-phosphate (MI1P) synthase (MI1PS) was introduced into Bacillus subtilis to convert glucose-6-phosphate (G6P) into MI1P. We found that inactivation of pbuE elevated intracellular concentrations of NAD+·NADH as an essential cofactor of MI1PS and was required to activate MI1PS. MI1P thus produced was dephosphorylated into myo-inositol by an intrinsic inositol monophosphatase, YktC, which was subsequently isomerized into scyllo-inositol via a previously established artificial pathway involving two inositol dehydrogenases, IolG and IolW. In addition, both glcP and glcK were overexpressed to feed more G6P and accelerate scyllo-inositol production. Consequently, a B. subtilis cell factory was demonstrated to produce 2 g L−1 scyllo-inositol from 20 g L−1 glucose. This cell factory provides an inexpensive way to produce scyllo-inositol, which will help us to challenge the growing problem of Alzheimer's disease in our aging society.
カテゴリ
科学技術イノベーション研究科
先端融合研究環
学術雑誌論文
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© The Author(s) 2020.
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
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journal article
言語
English (英語)
eISSN
2399-3642
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DOI
https://doi.org/10.1038/s42003-020-0814-7
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