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https://hdl.handle.net/20.500.14094/90006998
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2024-04-27
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90006998 (fulltext)
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メタデータID
90006998
アクセス権
open access
出版タイプ
Version of Record
タイトル
Analysis of Environmental DNA and Edaphic Factors for the Detection of the Snail Intermediate Host Oncomelania hupensis quadrasi
著者
Calata, Fritz Ivy C. ; Caranguian, Camille Z. ; Mendoza, Jillian Ela M. ; Fornillos, Raffy Jay C. ; Tabios, Ian Kim B. ; Fontanilla, Ian Kendrich C. ; Leonardo, Lydia R. ; Sunico, Louie S. ; Kawai, Satoru ; Chigusa, Yuichi ; Kikuchi, Mihoko ; Sato, Megumi ; Minamoto, Toshifumi ; Baoanan, Zenaida G. ; Sato, Marcello Otake
著者名
Calata, Fritz Ivy C.
著者名
Caranguian, Camille Z.
著者名
Mendoza, Jillian Ela M.
著者名
Fornillos, Raffy Jay C.
著者名
Tabios, Ian Kim B.
著者名
Fontanilla, Ian Kendrich C.
著者名
Leonardo, Lydia R.
著者名
Sunico, Louie S.
著者名
Kawai, Satoru
著者名
Chigusa, Yuichi
著者名
Kikuchi, Mihoko
著者名
Sato, Megumi
著者ID
A0574
研究者ID
1000050450656
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=481adea9a93d8196520e17560c007669
著者名
Minamoto, Toshifumi
源, 利文
ミナモト, トシフミ
所属機関名
人間発達環境学研究科
著者名
Baoanan, Zenaida G.
著者名
Sato, Marcello Otake
収録物名
Pathogens
巻(号)
8(4)
ページ
160-160
出版者
MDPI
刊行日
2019-12
公開日
2020-04-03
抄録
Background: The perpetuation of schistosomiasis japonica in the Philippines depends to a major extent on the persistence of its intermediate host Oncomelania hupensis quadrasi, an amphibious snail. While the malacological survey remains the method of choice in determining the contamination of the environment as evidenced by snails infected with schistosome larval stages, an emerging technology known as environmental DNA (eDNA) detection provides an alternative method. Previous reports showed that O. hupensis quadrasi eDNA could be detected in water, but no reports have been made on its detection in soil. Methods: This study, thus focused on the detection of O. hupensis quadrasi eDNA from soil samples collected from two selected schistosomiasis-endemic barangays in Gonzaga, Cagayan Valley using conventional and TaqMan-quantitative (qPCR) PCRs. Results: The results show that qPCR could better detect O. hupensis quadrasi eDNA in soil than the conventional method. In determining the possible distribution range of the snail, basic edaphic factors were measured and correlated with the presence of eDNA. The eDNA detection probability increases as the pH, phosphorous, zinc, copper, and potassium content increases, possibly indicating the conditions in the environment that favor the presence of the snails. A map was generated to show the probable extent of the distribution of the snails away from the body of the freshwater. Conclusion: The information generated from this study could be used to determine snail habitats that could be possible hotspots of transmission and should, therefore, be targeted for snail control or be fenced off from human and animal contact or from the contamination of feces by being a dumping site for domestic wastes.
キーワード
Oncomelania hupensis quadrasi
schistosomiasis japonica
environmental DNA
edaphic factors
snail surveillance
カテゴリ
人間発達環境学研究科
学術雑誌論文
権利
© 2019 by the authors. Licensee MDPI, Basel, Switzerland.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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資源タイプ
journal article
言語
English (英語)
eISSN
2076-0817
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関連情報
DOI
https://doi.org/10.3390/pathogens8040160
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