神戸大学附属図書館デジタルアーカイブ
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https://hdl.handle.net/20.500.14094/90007000
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2024-04-26
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90007000 (fulltext)
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メタデータID
90007000
アクセス権
open access
出版タイプ
Version of Record
タイトル
Detection of Schistosoma japonicum and Oncomelania hupensis quadrasi environmental DNA and its potential utility to schistosomiasis japonica surveillance in the Philippines
著者
Fornillos, Raffy Jay C. ; Sato, Marcello Otake ; Tabios, Ian Kim B. ; Sato, Megumi ; Leonardo, Lydia R. ; Chigusa, Yuichi ; Minamoto, Toshifumi ; Kikuchi, Mihoko ; Legaspi, Emelda R. ; Fontanilla, Ian Kendrich C.
著者名
Fornillos, Raffy Jay C.
著者名
Sato, Marcello Otake
著者名
Tabios, Ian Kim B.
著者名
Sato, Megumi
著者名
Leonardo, Lydia R.
著者名
Chigusa, Yuichi
著者ID
A0574
研究者ID
1000050450656
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=481adea9a93d8196520e17560c007669
著者名
Minamoto, Toshifumi
源, 利文
ミナモト, トシフミ
所属機関名
人間発達環境学研究科
著者名
Kikuchi, Mihoko
著者名
Legaspi, Emelda R.
著者名
Fontanilla, Ian Kendrich C.
収録物名
PLoS ONE
巻(号)
14(11)
ページ
e0224617-e0224617
出版者
Public Library of Science
刊行日
2019-11-20
公開日
2020-04-03
抄録
In recent years, the prevalence and infection intensity of Schistosoma japonicum in endemic areas of the Philippines have significantly decreased due to yearly population-based treatment strategies, yet transmission rates remain high and uninterrupted. An important indicator of active disease transmission is the presence of Schistosoma japonicum and its snail intermediate host Oncomelania hupensis quadrasi in freshwater habitats. In this study, we sought to apply a species-specific real-time PCR (qPCR) assay for the detection of S. japonicum and O. hupensis quadrasi in freshwater samples using environmental DNA approach that can complement the commonly utilized malacological survey in determining potential transmission foci in order to have a more effective snail surveillance strategy for schistosomiasis japonica in endemic areas. The newly developed assay was specific to S. japonicum and O. hupensis quadrasi with no amplification detected against non-target trematode Fasciola spp. and snails such as Lymnaea spp., Pomacea canaliculata, and Melanoides spp. that typically co-exist in the same environment. The assay effectiveness was determined using 19 environmental water samples collected from Northern Samar (N = 5 sites), Leyte (N = 11 sites) and Compostela Valley (N = 3 sites) and compared to malacological survey for determining O. hupensis quadrasi snail colonies and snail crushing to visualize S. japonicum cercariae. TaqMan qPCR targeting a short fragment of the cytochrome c oxidase subunit 1 (cox1) gene was positive for S. japonicum in 9 sites, for O. hupensis quadrasi in 9 sites, and for both S. japonicum and O. hupensis quadrasi in 5 sampling sites. Moreover, it was able to detect O. hupensis quadrasi in 3 out of 12 sites found negative and 6 out of 7 sites found positive through malacological survey, and in 4 of the 5 snail sites positive for snails with cercariae. Overall, this method can complement malacological surveys for monitoring of schistosomes in endemic areas of the Philippines, especially those with high risk of human infection.
カテゴリ
人間発達環境学研究科
学術雑誌論文
権利
© 2019 Fornillos et al.
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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資源タイプ
journal article
言語
English (英語)
eISSN
1932-6203
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関連情報
DOI
https://doi.org/10.1371/journal.pone.0224617
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