神戸大学附属図書館デジタルアーカイブ
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https://hdl.handle.net/20.500.14094/90007248
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2024-04-19
14:36 集計
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90007248 (fulltext)
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メタデータID
90007248
アクセス権
open access
出版タイプ
Version of Record
タイトル
Inhibitory Effects of Short-Chain Fatty Acids and ω-3 Polyunsaturated Fatty Acids on Profibrotic Factors in Dermal Fibroblasts
著者
Maeshige, Noriaki ; Torii, Kazuhiro ; Tabuchi, Hiroto ; Imai, Midori ; Koga, Yuka ; Uemura, Mikiko ; Aoyama-Ishikawa, Michiko ; Miyoshi, Makoto ; Fujino, Hidemi ; Terashi, Hiroto ; Usami, Makoto
著者ID
A1349
研究者ID
1000090617838
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=bad2e50ccfd61e15520e17560c007669
著者名
Maeshige, Noriaki
前重, 伯壮
マエシゲ, ノリアキ
所属機関名
保健学研究科
著者名
Torii, Kazuhiro
著者名
Tabuchi, Hiroto
著者名
Imai, Midori
著者名
Koga, Yuka
著者名
Uemura, Mikiko
著者名
Aoyama-Ishikawa, Michiko
著者ID
A1347
研究者ID
1000050433389
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=358199e26bf9770d520e17560c007669
著者名
Miyoshi, Makoto
三好, 真琴
ミヨシ, マコト
所属機関名
保健学研究科
著者ID
A1223
研究者ID
1000020278998
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=becd953e41b2f244520e17560c007669
著者名
Fujino, Hidemi
藤野, 英己
フジノ, ヒデミ
所属機関名
保健学研究科
著者ID
A0056
研究者ID
1000080217421
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=1431d96c128be803520e17560c007669
著者名
Terashi, Hiroto
寺師, 浩人
テラシ, ヒロト
所属機関名
医学部附属病院
著者名
Usami, Makoto
収録物名
ePlasty
巻(号)
19(e4)
ページ
25-39
出版者
Open Science Company
刊行日
2019-03-01
公開日
2020-07-16
抄録
Objective: Dermal fibroproliferative disorders impair patients’ quality of life. Although several therapeutic approaches exist for treatment of dermal scars, the development of effective ointments with few adverse effects could improve these therapeutic methods. Short-chain and ω-3 polyunsaturated fatty acids are reported to be immunomodulators with anti-inflammatory properties. Our aim was to evaluate anti-inflammatory and antifibrogenic effects of these fatty acids in human dermal fibroblasts. Methods: Cells were incubated with short-chain fatty acids (butyrate or propionate; 0-16 mM) and/or ω-3 polyunsaturated fatty acids (docosahexaenoic acid or eicosapentaenoic acid; 0-100 μM) for 24 hours to evaluate antifibrogenic effects and for 3 or 48 hours to evaluate anti-inflammatory effects after stimulation with lipopolysaccharide or without stimulation. Expression levels of α-smooth muscle actin, collagen I, collagen III, and IL-6 were evaluated, as were cell proliferation, stress fiber formation, and histone acetylation. Results: In the lipopolysaccharide-unstimulated group, butyrate inhibited mRNA expression of α-smooth muscle actin and collagen III more effectively than propionate and increased histone acetylation. Docosahexaenoic acid inhibited mRNA expression of α-smooth muscle actin and collagen III, whereas eicosapentaenoic acid did not. Combining butyrate with docosahexaenoic acid had stronger effects, downregulating α-smooth muscle actin, collagen I, and collagen III mRNA. As for cell proliferation and stress fiber formation, butyrate acted as a stronger inhibitor than docosahexaenoic acid and the combined administration had stronger effects. In the lipopolysaccharide-stimulated group, butyrate and docosahexaenoic acid attenuated IL-6 mRNA upregulation by lipopolysaccharide. Conclusion: Butyrate and docosahexaenoic acid may be a novel therapeutic approach to treatment of dermal fibroproliferative disorders.
キーワード
ω-3 polyunsaturated fatty acid
short-chain fatty acid
dermal fibroblast
pro-fibrotic factor
proinflammatory factor
カテゴリ
医学部附属病院
保健学研究科
学術雑誌論文
権利
© 2019 The Author(s).
This is an open-access article whereby the authors retain copyright of the work. The article is distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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資源タイプ
journal article
言語
English (英語)
eISSN
1937-5719
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