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https://hdl.handle.net/20.500.14094/90007258
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2024-04-20
10:36 集計
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90007258 (fulltext)
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メタデータID
90007258
アクセス権
open access
出版タイプ
Version of Record
タイトル
Cell Stress Reduction by a Novel Perfusion-Culture System Using Commercial Culture Dish
著者
Yasuda, Reiko ; Adachi, Shungo ; Okonogi, Atsuhito ; Anzai, Yohei ; Kamiyama, Tadataka ; Katano, Keiji ; Hoshi, Nobuhiko ; Mogi, Katsuo ; Natsume, Tohru
著者名
Yasuda, Reiko
著者名
Adachi, Shungo
著者名
Okonogi, Atsuhito
著者名
Anzai, Yohei
著者名
Kamiyama, Tadataka
著者名
Katano, Keiji
著者ID
A0039
研究者ID
1000010209223
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=fa741cc09ab44f05520e17560c007669
著者名
Hoshi, Nobuhiko
星, 信彦
ホシ, ノブヒコ
所属機関名
農学研究科
著者名
Mogi, Katsuo
著者名
Natsume, Tohru
収録物名
Applied Sciences
巻(号)
10(1)
ページ
95-95
出版者
MDPI
刊行日
2020-01
公開日
2020-07-17
抄録
Two typical medium replacement methods are employed to culture cells, that is, traditional manual medium change, wherein complete medium changes are performed at short-term intervals; and perfusion, wherein continuous culture media delivery and discharge are performed. The former is associated with the issue of readily accumulation of metabolic waste products, which is resolved by the latter. However, the latter requires a specific chamber for cell culturing such as a microfluidic channel for flow stability. Generally, bio-medical researchers require a culturing process that utilizes commercial culture dishes to test their conventional manipulation know-how, experience, and protocols. Thus, we constructed a perfusion-culture system using commercial 35 mm culture dishes. To use commercial culture dishes, it is necessary to maintain the culturing conditions including a constant volume and uniform flow rate, and we invented a novel adapter, culture dish adapter (CD-Adapter), attached to a commercial culture dish. The CD-Adapter is fixed with a holding jig to keep the medium volume constant, so it can maintain a uniform flow in the culture dish. Additionally, we demonstrate the applicability of the system by applying it to a perfusion culture of 293T cells, revealing a 50% reduction in DDIT3, a cellular stress marker, compared with that in conventional manual medium change.
キーワード
manual medium change
perfusion culture
cellular stress
cell culture system
microfluidic device
カテゴリ
農学研究科
学術雑誌論文
権利
© 2019 by the authors. Licensee MDPI, Basel, Switzerland.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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資源タイプ
journal article
言語
English (英語)
eISSN
2076-3417
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関連情報
DOI
https://doi.org/10.3390/app10010095
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