神戸大学附属図書館デジタルアーカイブ
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https://hdl.handle.net/20.500.14094/90007280
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2024-04-20
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90007280 (fulltext)
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メタデータID
90007280
アクセス権
open access
出版タイプ
Version of Record
タイトル
Establishment of an organ culture system to induce Sertoli cell differentiation from undifferentiated mouse gonads
著者
Hasegawa, Chinatsu ; Yokoyama, Toshifumi ; Umemura, Yuria ; Kawanishi, Kohei ; Miura, Yuuka ; Takada, Nanako ; Ohno, Shuji ; Onaru, Kanoko ; Omotehara, Takuya ; Hirano, Tetsushi ; Mantani, Yohei ; Miki, Takanori ; Hoshi, Nobuhiko
著者名
Hasegawa, Chinatsu
著者ID
A0061
研究者ID
1000010380156
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=158c55140a1540e9520e17560c007669
著者名
Yokoyama, Toshifumi
横山, 俊史
ヨコヤマ, トシフミ
所属機関名
農学研究科
著者名
Umemura, Yuria
著者名
Kawanishi, Kohei
著者名
Miura, Yuuka
著者名
Takada, Nanako
著者名
Ohno, Shuji
著者名
Onaru, Kanoko
著者名
Omotehara, Takuya
著者名
Hirano, Tetsushi
著者ID
A1072
研究者ID
1000030724984
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=baf1f569c1518783520e17560c007669
著者名
Mantani, Yohei
万谷, 洋平
マンタニ, ヨウヘイ
所属機関名
農学研究科
著者名
Miki, Takanori
著者ID
A0039
研究者ID
1000010209223
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=fa741cc09ab44f05520e17560c007669
著者名
Hoshi, Nobuhiko
星, 信彦
ホシ, ノブヒコ
所属機関名
農学研究科
収録物名
Journal of Veterinary Medical Science
巻(号)
82(4)
ページ
414-421
出版者
Japanese Society of Veterinary Science
刊行日
2020-04
公開日
2020-07-21
抄録
Organ culture systems are useful for elucidating the process of testicular differentiation from mammalian undifferentiated genetically male gonads, as they permit various experiments, including experiments involving the control of gene expression. However, without addition of testicular differentiation-related factors, it is difficult to induce the formation of testis cord from immature gonads by a time point earlier 12 tail somites (ts) that corresponding to 11.0 days post coitum (dpc). In this study, we attempted to establish an organ culture system that induces testis formation from immature gonads (around 8 ts: 10.5 dpc) just before Sry (sex-determining region of the Y chromosome) expression. A paired gonad-mesonephros complex of around 8 ts was placed in the groove of an agarose gel block and put the semi-cylindrical piece of agarose gel to maintain the gonad morphology. The gonads were cultured in the gas phase for 96 hr. As a result, testis cord-like structures appeared in many genetically male gonads. Cells expressing the Sertoli cell markers Sox9 (SRY-box 9) and Amh (anti-Müllerian hormone) were observed, while granulosa cell marker Foxl2 (forkhead box L2) was not detected. In addition, Sox9-and Amh-expressing cells were observed throughout the entire gonad in many individuals. Amh mRNA expression was also upregulated. Surprisingly, formation of a partial testicular structure was observed from more immature gonads (6 ts). These results show that our gonadal organ culture system is useful for elucidating the regulation mechanism of Sry expression in undifferentiated bipotential gonads.
キーワード
Amh (anti-Mullerian hormone)
organ culture
Sertoli cell
testis differentiation
undifferentiated gonad
カテゴリ
農学研究科
学術雑誌論文
権利
©2020 The Japanese Society of Veterinary Science.
This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd)License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
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資源タイプ
journal article
言語
English (英語)
ISSN
0916-7250
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eISSN
1347-7439
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NCID
AA11510037
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関連情報
DOI
https://doi.org/10.1292/jvms.20-0036
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