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https://hdl.handle.net/20.500.14094/90007417
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2024-04-27
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90007417 (fulltext)
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メタデータID
90007417
アクセス権
open access
出版タイプ
Version of Record
タイトル
Digital Holographic Multimodal Cross-Sectional Fluorescence and Quantitative Phase Imaging System
著者
Kumar, Manoj ; Quan, Xiangyu ; Awatsuji, Yasuhiro ; Tamada, Yosuke ; Matoba, Osamu
著者名
Kumar, Manoj
著者ID
A2220
研究者ID
1000040814778
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=82a31abecb087f7e520e17560c007669
著者名
Quan, Xiangyu
ゼン, コウギョク
所属機関名
システム情報学研究科
著者名
Awatsuji, Yasuhiro
著者名
Tamada, Yosuke
著者ID
A0303
研究者ID
1000020282593
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=ed1b78f0382a9921520e17560c007669
著者名
Matoba, Osamu
的場, 修
マトバ, オサム
所属機関名
先端融合研究環
収録物名
Scientific Reports
巻(号)
10(1)
ページ
7580-7580
出版者
Nature Research
刊行日
2020-05-15
公開日
2020-09-04
抄録
We present a multimodal imaging system based on simple off-axis digital holography, for simultaneous recording and retrieval of cross-sectional fluorescence and quantitative phase imaging of the biological specimen. Synergism in the imaging capabilities can be achieved by incorporating two off-axis digital holographic microscopes integrated to record different information at the same time. The cross-sectional fluorescence imaging is realized by a common-path configuration of the single-shot off-axis incoherent digital holographic system. The quantitative phase imaging, on the other hand, is achieved by another off-axis coherent digital holographic microscopy operating in transmission mode. The fundamental characteristics of the proposed multimodal system are confirmed by performing various experiments on fluorescent beads and fluorescent protein-labeled living cells of the moss Physcomitrella patens lying at different axial depth positions. Furthermore, the cross-sectional live fluorescence and phase imaging of the fluorescent beads are demonstrated by the proposed multimodal system. The experimental results presented here corroborate the feasibility of the proposed system and indicate its potential in the applications to analyze the functional and structural behavior of biological cells and tissues.
カテゴリ
システム情報学研究科
先端融合研究環
学術雑誌論文
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© The Author(s) 2020.
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
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資源タイプ
journal article
言語
English (英語)
eISSN
2045-2322
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関連情報
DOI
https://doi.org/10.1038/s41598-020-64028-x
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