神戸大学附属図書館デジタルアーカイブ
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https://hdl.handle.net/20.500.14094/90007520
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2024-04-27
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90007520 (fulltext)
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メタデータID
90007520
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open access
出版タイプ
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タイトル
Aqp9 Gene Deletion Enhances Retinal Ganglion Cell (RGC) Death and Dysfunction Induced by Optic Nerve Crush: Evidence that Aquaporin 9 Acts as an Astrocyte-to-Neuron Lactate Shuttle in Concert with Monocarboxylate Transporters To Support RGC Function and Survival
著者
Mori, Sotaro ; Kurimoto, Takuji ; Miki, Akiko ; Maeda, Hidetaka ; Kusuhara, Sentaro ; Nakamura, Makoto
著者名
Mori, Sotaro
著者ID
A1535
研究者ID
1000050388815
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=09106101bc83c492520e17560c007669
著者名
Kurimoto, Takuji
栗本, 拓治
クリモト, タクジ
所属機関名
医学研究科
著者ID
A1504
研究者ID
1000010726988
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=704fe343d155a8da520e17560c007669
著者名
Miki, Akiko
三木, 明子
ミキ, アキコ
所属機関名
医学部附属病院
著者名
Maeda, Hidetaka
著者ID
A1380
研究者ID
1000040437463
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=32b946773fbb21da520e17560c007669
著者名
Kusuhara, Sentaro
楠原, 仙太郎
クスハラ, センタロウ
所属機関名
医学研究科
著者ID
A0882
研究者ID
1000080273788
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=82142348522a81ad520e17560c007669
著者名
Nakamura, Makoto
中村, 誠
ナカムラ, マコト
所属機関名
医学研究科
収録物名
Molecular Neurobiology
巻(号)
57(11)
ページ
4530-4548
出版者
Springer
刊行日
2020-11
公開日
2020-10-12
抄録
Aquaporin 9 (AQP9) is an aquaglyceroporin that can transport lactate. Accumulating evidence suggests that astrocyte-to-neuron lactate shuttle (ANLS) plays a critical role in energy metabolism in neurons, including retinal ganglion cells (RGCs). To test the hypothesis that AQP9, in concert with monocarboxylate transporters (MCTs), participates in ANLS to maintain function and survival of RGCs, Aqp9-null mice and wild-type (WT) littermates were subjected to optic nerve crush (ONC) with or without intravitreal injection of an MCT2 inhibitor. RGC density was similar between the Aqp9-null mice and WT mice without ONC, while ONC resulted in significantly more RGC density reduction in the Aqp9-null mice than in the WT mice at day 7. Positive scotopic threshold response (pSTR) amplitude values were similar between the two groups without ONC, but were significantly more reduced in the Aqp9-null mice than in the WT mice 7days after ONC. MCT2 inhibitor injection accelerated RGC death and pSTR amplitude reduction only in the WT mice with ONC. Immunolabeling revealed that both RGCs and astrocytes expressed AQP9, that ONC predominantly reduced astrocytic AQP9 expression, and that MCTs 1, 2, and 4 were co-localized with AQP9 at the ganglion cell layer. These retinal MCTs were also co-immunoprecipitated with AQP9 in the WT mice. ONC decreased the co-immunoprecipitation of MCTs 1 and 4, but did not impact co-immunoprecipitation of MCT2. Retinal glucose transporter 1 expression was increased in Aqp9-null mice. Aqp9 gene deletion reduced and increased the intraretinal l-lactate and d-glucose concentrations, respectively. Results suggest that AQP9 acts as the ANLS to maintain function and survival of RGCs.
キーワード
Aquaporin 9
Monocarboxylate transporter
Astrocyte-to-neuron lactate shuttle
Optic nerve crush
Retinal ganglion cells
Scotopic threshold response
カテゴリ
医学研究科
医学部附属病院
学術雑誌論文
権利
© The Author(s) 2020.
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
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資源タイプ
journal article
言語
English (英語)
ISSN
0893-7648
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eISSN
1559-1182
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NCID
AA10680654
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関連情報
DOI
https://doi.org/10.1007/s12035-020-02030-0
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