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https://hdl.handle.net/20.500.14094/90007537
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2024-04-26
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90007537 (fulltext)
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メタデータID
90007537
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open access
出版タイプ
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タイトル
Metabolic Engineering of Shikimic Acid-Producing Corynebacterium glutamicum From Glucose and Cellobiose Retaining Its Phosphotransferase System Function and Pyruvate Kinase Activities
著者
Sato, Naoki ; Kishida, Mayumi ; Nakano, Mariko ; Hirata, Yuuki ; Tanaka, Tsutomu
著者名
Sato, Naoki
著者名
Kishida, Mayumi
著者名
Nakano, Mariko
著者名
Hirata, Yuuki
著者ID
A0311
研究者ID
1000090436551
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=106238a6648c3bb7520e17560c007669
著者名
Tanaka, Tsutomu
田中, 勉
タナカ, ツトム
所属機関名
工学研究科
収録物名
Frontiers in Bioengineering and Biotechnology
巻(号)
8
ページ
569406-569406
出版者
Frontiers Media
刊行日
2020-09-10
公開日
2020-10-19
抄録
The production of aromatic compounds by microbial production is a promising and sustainable approach for producing biomolecules for various applications. We describe the metabolic engineering of Corynebacterium glutamicum to increase its production of shikimic acid. Shikimic acid and its precursor-consuming pathways were blocked by the deletion of the shikimate kinase, 3-dehydroshikimate dehydratase, shikimate dehydratase, and dihydroxyacetone phosphate phosphatase genes. Plasmid-based expression of shikimate pathway genes revealed that 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase, encoded by aroG, and DHQ synthase, encoded by aroB, are key enzymes for shikimic acid production in C. glutamicum. We constructed a C. glutamicum strain with aroG, aroB and aroE3 integrated. This strain produced 13.1 g/L of shikimic acid from 50 g/L of glucose, a yield of 0.26 g-shikimic acid/g-glucose, and retained both its phosphotransferase system and its pyruvate kinase activity. We also endowed β-glucosidase secreting ability to this strain. When cellobiose was used as a carbon source, the strain produced shikimic acid at 13.8 g/L with the yield of 0.25 g-shikimic acid/g-glucose (1 g of cellobiose corresponds to 1.1 g of glucose). These results demonstrate the feasibility of producing shikimic acid and its derivatives using an engineered C. glutamicum strain from cellobiose as well as glucose.
キーワード
shikimic acid
shikimate pathway
Corynebacterium glutamicum
metabolic engineering
beta-glucosidase
cellobiose
カテゴリ
工学研究科
学術雑誌論文
権利
© 2020 Sato, Kishida, Nakano, Hirata and Tanaka.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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資源タイプ
journal article
言語
English (英語)
eISSN
2296-4185
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関連情報
DOI
https://doi.org/10.3389/fbioe.2020.569406
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