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https://hdl.handle.net/20.500.14094/90007892
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2024-04-24
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90007892 (fulltext)
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メタデータID
90007892
アクセス権
open access
出版タイプ
Version of Record
タイトル
Seasonal monitoring of Hida salamander Hynobius kimurae using environmental DNA with a genus-specific primer set
著者
Jo, Toshiaki ; Tomita, Sei ; Kohmatsu, Yukihiro ; Osathanunkul, Maslin ; Ushimaru, Atushi ; Minamoto, Toshifumi
著者名
Jo, Toshiaki
著者名
Tomita, Sei
著者名
Kohmatsu, Yukihiro
著者名
Osathanunkul, Maslin
著者ID
A0565
研究者ID
1000070399327
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=0eeeff0906044ae4520e17560c007669
著者名
Ushimaru, Atushi
丑丸, 敦史
ウシマル, アツシ
所属機関名
人間発達環境学研究科
著者ID
A0574
研究者ID
1000050450656
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=481adea9a93d8196520e17560c007669
著者名
Minamoto, Toshifumi
源, 利文
ミナモト, トシフミ
所属機関名
人間発達環境学研究科
収録物名
Endangered Species Research
巻(号)
43
ページ
341-352
出版者
Inter-Research Science Publisher
刊行日
2020
公開日
2021-03-05
抄録
The diversity and the abundance of amphibians have dramatically declined globally over the past 30 years, and the monitoring and conservation of their habitats is essential. However, traditional methods such as bait trapping and mark-recapture are costly, and morphological identification usually requires a high level of taxonomic expertise. Here, seasonal surveillances of Hida salamander Hynobius kimurae were performed by means of environmental DNA (eDNA) analysis with Hynobius-specific primers and a species-specific TaqMan probe. Water sampling and visual surveys were conducted seasonally in a stream in Kyoto Prefecture, Japan. Detection rates of eDNA were then calculated by real-time PCR, and eDNA site occupancy probability was estimated by multi-scale occupancy modeling. The eDNA-based detection rate of Hida salamander was 76.7%, whereas the visual survey-based detection rate was 23.3%, and target eDNA was detected at almost all sites where the presence of target species was visually confirmed. Moreover, factors relating to the site- and sample-level occurrence probabilities of the target eDNA differed depending on the developmental stage of the target species. Our findings support previous studies showing that eDNA analysis enables an effective assessment of amphibian distributions without damaging the organisms or their habitat, and we compare for the first time the site occupancy probability of amphibian eDNA throughout the life cycle of an amphibian species. The present study contributes to the development of eDNA analysis as a tool for understanding the distribution and seasonal activity of amphibian species and will thus aid in the planning of conservation measures and habitat restoration for these species.
キーワード
Amphibian
Conservation management
Environmental DNA (eDNA)
Hynobius kimurae
Real-time PCR
Spatiotemporal ecology
カテゴリ
人間発達環境学研究科
学術雑誌論文
権利
© The authors 2020.
Open Access under Creative Commons by Attribution Licence. Use, distribution and reproduction are un-restricted. Authors and original publication must be credited.
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資源タイプ
journal article
言語
English (英語)
ISSN
1863-5407
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eISSN
1613-4796
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NCID
AA12186965
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関連情報
DOI
https://doi.org/10.3354/esr01073
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