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https://hdl.handle.net/20.500.14094/90008054
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2024-04-26
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90008054 (fulltext)
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メタデータID
90008054
アクセス権
open access
出版タイプ
Version of Record
タイトル
Detection of herbivory: eDNA detection from feeding marks on leaves
著者
Kudoh, Aoi ; Minamoto, Toshifumi ; Yamamoto, Satoshi
著者名
Kudoh, Aoi
著者ID
A0574
研究者ID
1000050450656
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=481adea9a93d8196520e17560c007669
著者名
Minamoto, Toshifumi
源, 利文
ミナモト, トシフミ
所属機関名
人間発達環境学研究科
著者名
Yamamoto, Satoshi
収録物名
Environmental DNA
巻(号)
2(4)
ページ
627-634
出版者
Wiley
刊行日
2020-10
公開日
2021-03-31
抄録
Many techniques have been developed to investigate the interactions between plants and herbivorous insects in natural environments and are generally used to determine either (a) which plant species are eaten by a specific herbivorous insect or (b) which herbivorous insect species are herbivores of a specific plant. The former problem is usually addressed by the direct observation of feeding and microscopic observation of gut contents and excrements, as well as the application of DNA‐barcoding techniques. However, the latter problem has typically been addressed using time‐consuming methods, such as direct observation and rearing. Therefore, more efficient techniques are needed for identifying and quantifying the interactions of plants with herbivorous insects. The present study demonstrates that the environmental DNA (eDNA) of herbivorous insects can be recovered from leaves with external foliage feeding marks. Mitochondrial DNA fragments of herbivorous insects were detected from insect‐exposed leaves using primer sets that amplified the DNA of target species. The amplification rate of the herbivorous insect DNA was positively associated with the rim length of feeding marks, which suggests that most of the insect DNA came from the feeding marks. Additionally, we showed that this method has the potential to detect eDNA from field‐collected leaves. This time‐efficient approach will contribute to the detection of plant–insect herbivore interactions.
キーワード
herbivory
insect
larva
leaf
real‐time PCR
saliva
カテゴリ
人間発達環境学研究科
学術雑誌論文
権利
© 2020 The Authors. Environmental DNA published by John Wiley & Sons Ltd.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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資源タイプ
journal article
言語
English (英語)
eISSN
2637-4943
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関連情報
DOI
https://doi.org/10.1002/edn3.113
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