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https://hdl.handle.net/20.500.14094/90008156
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2024-04-21
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90008156 (fulltext)
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90008156
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open access
出版タイプ
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タイトル
Model Lipid Membranes Assembled from Natural Plant Thylakoids into 2D Microarray Patterns as a Platform to Assess the Organization and Photophysics of Light-Harvesting Proteins
著者
Meredith, Sophie A. ; Yoneda, Takuro ; Hancock, Ashley M. ; Connell, Simon D. ; Evans, Stephen D. ; Morigaki, Kenichi ; Adams, Peter G.
著者名
Meredith, Sophie A.
著者名
Yoneda, Takuro
著者名
Hancock, Ashley M.
著者名
Connell, Simon D.
著者名
Evans, Stephen D.
著者ID
A1236
研究者ID
1000010358179
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=f6c039bc884596f8520e17560c007669
著者名
Morigaki, Kenichi
森垣, 憲一
モリガキ, ケンイチ
所属機関名
バイオシグナル総合研究センター
著者名
Adams, Peter G.
収録物名
Small
巻(号)
17(14)
ページ
2006608
出版者
Wiley-VCH
刊行日
2021-04-08
公開日
2021-04-20
抄録
Natural photosynthetic "thylakoid" membranes found in green plants contain a large network of light-harvesting (LH) protein complexes. Rearrangement of this photosynthetic machinery, laterally within stacked membranes called "grana", alters protein-protein interactions leading to changes in the energy balance within the system. Preparation of an experimentally accessible model system that allows the detailed investigation of these complex interactions can be achieved by interfacing thylakoid membranes and synthetic lipids into a template comprised of polymerized lipids in a 2D microarray pattern on glass surfaces. This paper uses this system to interrogate the behavior of LH proteins at the micro- and nanoscale and assesses the efficacy of this model. A combination of fluorescence lifetime imaging and atomic force microscopy reveals the differences in photophysical state and lateral organization between native thylakoid and hybrid membranes, the mechanism of LH protein incorporation into the developing hybrid membranes, and the nanoscale structure of the system. The resulting model system within each corral is a high-quality supported lipid bilayer that incorporates laterally mobile LH proteins. Photosynthetic activity is assessed in the hybrid membranes versus proteoliposomes, revealing that commonly used photochemical assays to test the electron transfer activity of photosystem II may actually produce false-positive results.
キーワード
artificial photosynthesis
atomic force microscopy (AFM)
biohybrids
chlorophyll fluorescence
fluorescence lifetime imaging microscopy (FLIM)
light‐
harvesting
supported lipid bilayers
カテゴリ
バイオシグナル総合研究センター
学術雑誌論文
権利
© 2021 The Authors. Small published by Wiley‐VCH GmbH.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited
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資源タイプ
journal article
言語
English (英語)
ISSN
1613-6810
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eISSN
1613-6829
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NCID
AA11975212
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関連情報
DOI
https://doi.org/10.1002/smll.202006608
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