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https://hdl.handle.net/20.500.14094/90008202
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2024-04-19
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90008202 (fulltext)
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90008202
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open access
出版タイプ
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タイトル
Molecular organization of recombinant human-Arabidopsis chromosomes in hybrid cell lines
著者
Liu, Yikun ; Liaw, Yeng Mun ; Teo, Chee How ; Capal, Petr ; Wada, Naoki ; Fukui, Kiichi ; Dolezel, Jaroslav ; Ohmido, Nobuko
著者名
Liu, Yikun
著者名
Liaw, Yeng Mun
著者名
Teo, Chee How
著者名
Capal, Petr
著者名
Wada, Naoki
著者名
Fukui, Kiichi
著者名
Dolezel, Jaroslav
著者ID
A0558
研究者ID
1000030343263
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=489af0c45732b381520e17560c007669
著者名
Ohmido, Nobuko
近江戸, 伸子
オオミド, ノブコ
所属機関名
人間発達環境学研究科
収録物名
Scientific Reports
巻(号)
11(1)
ページ
7160
出版者
Nature Portfolio
刊行日
2021-03-30
公開日
2021-04-27
抄録
Although plants and animals are evolutionarily distant, the structure and function of their chromosomes are largely conserved. This allowed the establishment of a human-Arabidopsis hybrid cell line in which a neo-chromosome was formed by insertion of segments of Arabidopsis chromosomes into human chromosome 15. We used this unique system to investigate how the introgressed part of a plant genome was maintained in human genetic background. The analysis of the neo-chromosome in 60- and 300-day-old cell cultures by next-generation sequencing and molecular cytogenetics suggested its origin by fusion of DNA fragments of different sizes from Arabidopsis chromosomes 2, 3, 4, and 5, which were randomly intermingled rather than joined end-to-end. The neo-chromosome harbored Arabidopsis centromeric repeats and terminal human telomeres. Arabidopsis centromere wasn't found to be functional. Most of the introgressed Arabidopsis DNA was eliminated during the culture, and the Arabidopsis genome in 300-day-old culture showed significant variation in copy number as compared with the copy number variation in the 60-day-old culture. Amplified Arabidopsis centromere DNA and satellite repeats were localized at particular loci and some fragments were inserted into various positions of human chromosome. Neo-chromosome reorganization and behavior in somatic cell hybrids between the plant and animal kingdoms are discussed.
カテゴリ
人間発達環境学研究科
学術雑誌論文
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© The Author(s) 2021. Open Access
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
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資源タイプ
journal article
言語
English (英語)
eISSN
2045-2322
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関連情報
DOI
https://doi.org/10.1038/s41598-021-86130-4
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