神戸大学附属図書館デジタルアーカイブ
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学内刊行物
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https://hdl.handle.net/20.500.14094/90008207
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22
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2024-04-26
16:00 集計
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90008207 (fulltext)
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メタデータID
90008207
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open access
出版タイプ
Version of Record
タイトル
Modularized microscope based on parallel phase-shifting digital holography for imaging of living biospecimens
著者
Inamoto, Junya ; Fukuda, Takahito ; Inoue, Tomoyoshi ; Shimizu, Kazuki ; Nishio, Kenzo ; Xia, Peng ; Matoba, Osamu ; Awatsuji, Yasuhiro
著者名
Inamoto, Junya
著者名
Fukuda, Takahito
著者名
Inoue, Tomoyoshi
著者名
Shimizu, Kazuki
著者名
Nishio, Kenzo
著者名
Xia, Peng
著者ID
A0303
研究者ID
1000020282593
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=ed1b78f0382a9921520e17560c007669
著者名
Matoba, Osamu
的場, 修
マトバ, オサム
所属機関名
先端融合研究環
著者名
Awatsuji, Yasuhiro
収録物名
Journal of Biomedical Optics
巻(号)
25(12)
ページ
123706
出版者
SPIE
刊行日
2020-12-04
公開日
2021-04-26
抄録
Significance: Parallel phase-shifting digital holographic microscope (PPSDHM) is powerful for three-dimensional (3D) measurements of dynamic specimens. However, the PPSDHM reported previously was directly fixed on the optical bench and imposed difficulties case, thus it is required to modify the specification of the microscope or transport the microscope to another location. Aim: We present a modularized PPSDHM. We construct the proposed PPSDHM and demonstrate the 3D measurement capability of the PPSDHM. Approach: The PPSDHM was designed as an inverted microscope to record transparent objects and modularized by integrating the optical elements of the PPSDHM on an optical breadboard. To demonstrate the effectiveness of the PPSDHM, we recorded a 3D motion-picture of moving Volvoxes at 1000 frames / s and carried out 3D tracking of the Volvoxes. Results: The PPSDHM was practically realized and 3D images of objects were successfully reconstructed from holograms recorded with a single-shot exposure. The 3D trajectories of Volvoxes were obtained from the reconstructed images. Conclusions: We established a modularized PPSDHM that is capable of 3D image acquisition by integrating the optical elements of the PPSDHM on an optical breadboard. The recording capability of 3D motion-pictures of dynamic specimens was experimentally demonstrated by the PPSDHM.
カテゴリ
先端融合研究環
学術雑誌論文
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©The Authors.
Published by SPIE under a Creative Commons Attribution 4.0 Unported License.
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資源タイプ
journal article
言語
English (英語)
eISSN
1560-2281
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関連情報
DOI
https://doi.org/10.1117/1.JBO.25.12.123706
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