神戸大学附属図書館デジタルアーカイブ
入力補助
English
カテゴリ
学内刊行物
ランキング
アクセスランキング
ダウンロードランキング
https://hdl.handle.net/20.500.14094/90008218
このアイテムのアクセス数:
44
件
(
2024-04-25
16:47 集計
)
閲覧可能ファイル
ファイル
フォーマット
サイズ
閲覧回数
説明
90008218 (fulltext)
pdf
818 KB
31
メタデータ
ファイル出力
メタデータID
90008218
アクセス権
open access
出版タイプ
Version of Record
タイトル
Messenger RNA typing of environmental RNA (eRNA): A case study on zebrafish tank water with perspectives for the future development of eRNA analysis on aquatic vertebrates
著者
Tsuri, Kenji ; Ikeda, Shizuya ; Hirohara, Takaya ; Shimada, Yasuhito ; Minamoto, Toshifumi ; Yamanaka, Hiroki
著者名
Tsuri, Kenji
著者名
Ikeda, Shizuya
著者名
Hirohara, Takaya
著者名
Shimada, Yasuhito
著者ID
A0574
研究者ID
1000050450656
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=481adea9a93d8196520e17560c007669
著者名
Minamoto, Toshifumi
源, 利文
ミナモト, トシフミ
所属機関名
人間発達環境学研究科
著者名
Yamanaka, Hiroki
収録物名
Environmental DNA
巻(号)
3(1)
ページ
14-21
出版者
Wiley
刊行日
2021-01
公開日
2021-04-27
抄録
As an alternative/supplement to conventional biodiversity survey methods, environmental DNA (eDNA) analysis has developed rapidly during the past decade, and is widely used for the biomonitoring of wildlife. However, the interpretation of eDNA results has been limited to the presence/absence or biomass/abundance of the target species because of uncertainty regarding the dynamics of eDNA in natural environments. This limitation may be ameliorated by targeting environmental RNA (eRNA). RNA is more prone to degrade than DNA and the pattern of messenger RNA (mRNA) expression changes depending on physiological conditions, meaning that the presence or concentration of mRNA could reflect the organism's presence with higher temporal resolution and provide information beyond simple presence/absence. Technical developments in the detection of eRNA focusing on mRNA with these distinct features could permit the advanced usage of genetic materials in water. In advancing this technique, we initiated this study asking that if we can detect elevated levels of eRNA whose genes are specific to a tissue source (e.g., gills or skin), then could not we infer the tissue origin of the genetic material detected. To this end, we developed gene‐specific primer sets for the target genes with biased expression in the gills, skin, and intestine, and conducted reverse transcription–polymerase chain reaction on zebrafish breeding tank water samples, obtaining positive results for all assays. The result of our experiment confirmed that the specific target tissues can be the source of genetic materials detected in water and with that we offer a proof of concept for eRNA analysis targeting specific mRNAs of aquatic vertebrates. In this commentary, we provide information on the experimental steps used for mRNA typing of eRNA from zebrafish as well as the limitations and challenges of this technique and the prospect of mRNA typing of eRNA in the future.
キーワード
biological monitoring
conservation of natural resources
gene expression
polymerase chain reaction
reverse transcription
RNA
カテゴリ
人間発達環境学研究科
学術雑誌論文
権利
© 2020 The Authors. Environmental DNA published by John Wiley & Sons Ltd.
This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
詳細を表示
資源タイプ
journal article
言語
English (英語)
eISSN
2637-4943
OPACで所蔵を検索
CiNiiで学外所蔵を検索
関連情報
DOI
https://doi.org/10.1002/edn3.169
ホームへ戻る